This product is specially developed for rapid PCR amplification of mouse tissues (mouse tail, mouse toe, mouse ear, etc.). It is equipped with a powerful tissue digesting solution, which can rapidly release sufficient genomic DNA from mouse tail and other tissues, and the product can be directly amplified by PCR without the need for overnight digestion, phenol-chloroform extraction, or column purification, which greatly shortens the time-consuming experiments, simplifies the process of operation, and reduces the chance of contamination. This greatly shortens the time required for the experiment, simplifies the operation process and reduces the chance of contamination.
The product also has high amplification efficiency and tolerance for simple or complex templates, short fragments or long fragments of PCR amplification, and can tolerate 20% mouse tail lysate templates.
The product has a fast extension speed and can obtain the results in a short time. For PCR reaction, just add template, primer and water to the premix for amplification; at the same time, the product also adds the color reagent required for electrophoresis detection, the solution is purple-red; after the PCR reaction is completed, it can be used for agarose gel electrophoresis directly. This premix solution is easy to operate and minimizes human error.
A monoclonal antibody that inhibits the activity of DNA polymerase at ambient temperature has been added to Hot Start PCR to inhibit primer dimer formation and non-specific amplification. The PCR product amplified by this product does not contain an A base at the 3’ end and therefore cannot be used directly for TA cloning.
1. Simple and fast: No need for time-consuming and cumbersome DNA purification steps, 5 min for fast DNA release.
2. Wide application: suitable for one-step extraction of crude cleavage templates from mouse tail, ear and toe tissues.
3. Efficient amplification: fast extension speed, the fastest amplification speed of fragments under 4 kb can reach 5 sec/kb.
4. Highly tolerant: greater inhibitor tolerance, up to 20% rough cleavage template.
| individual parts making up a compound | norm |
| 2X Mouse Tissue Fast PCR Master Mix (dye plus) | 500 μl × 6 pcs |
| Mouse Tissue Lysis Buffer | 12 ml |
| Proteinase K (20 mg/ml) | 120 μl |
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
