AdeptTect General purpose direct PCR premix (with dye)

This product is a 2-fold pre-mixed reagent for general-purpose direct PCR reaction, with strong amplification performance and wide applicability of templates, it can effectively amplify complex templates such as those with high GC content and high AT content. It is especially suitable for PCR amplification after direct or simple lysis of various types of samples, including animals, plants, fungi, bacteria, blood and cells, etc. No DNA purification step is required, which greatly shortens the length of experiments and saves costs. Simple cleavage can be done by alkaline cleavage or proteinase K cleavage. If proteinase K cleavage is chosen, our products Lysis Buffer for PCR (Code No. AG12306 and AG12307) can be used. Meanwhile, this product has a fast extension speed (~20 sec / kb), which can obtain the results in a short period of time.
For PCR reaction, just add the template, primer and water into the premix solution for amplification; at the same time, the color reagent required for electrophoresis detection is also added into this product, and the solution appears purple-red color; after the PCR reaction is completed, it can be used for agarose gel electrophoresis directly. This premix solution is easy to operate and minimizes human error.
A monoclonal antibody that inhibits the activity of DNA polymerase at room temperature has been added to this product to enable Hot Start PCR, which effectively inhibits the formation of primer dimers and non-specific amplification. The PCR product amplified by this product does not contain the A base at the 3’ end, so it cannot be used directly for TA cloning.

① This product has strong amplification performance and wide applicability of templates, which is suitable for PCR amplification of various types of samples after direct or simple lysis; it does not require DNA purification, which shortens the length of experiments and saves costs; it can amplify DNA fragments of up to 12 kb in length by directly using rice leaves as templates.
② This product is a 2-fold concentration of premixed solution, only need to add the template, primers and water for PCR amplification, easy to operate, reduce human error, greatly improve the efficiency of the experiment.
③ The product has a fast elongation speed (~20 sec / kb), which allows the test results to be obtained in a relatively short period of time.
④ This product contains violet dye, after PCR can be directly carried out agarose gel electrophoresis, without adding electrophoresis sample buffer, electrophoresis has a light violet-red indicator band.
⑤ The optimized reaction system is well adapted to PCR amplification of complex templates, and is effective for templates with high GC content and high AT content.

individual parts making up a compound	norm
2X Universal Direct PCR Master Mix (dye plus)	500 μl × 6 pcs

Storage temperature: -20℃
Transportation temperature: dry ice or -20℃ ice bag