Total RNA can be extracted from animal tissues, plant materials, various microorganisms, cultured cells, etc. The samples can be fully cleaved in RNAex, and after centrifugation with chloroform (or RNA extraction auxiliary reagent Code No. AG21303), the solution will form a supernatant layer, an intermediate layer, and an organic layer (a bright-red lower layer containing protein, polysaccharide, fatty acid, cell debris, and DNA), and the RNA will be distributed in the supernatant layer. After centrifugation, the solution will form a supernatant layer and an intermediate layer and an organic layer (bright red lower layer, containing proteins, polysaccharides, fatty acids, cellular debris, and DNA), and the RNA will be distributed in the supernatant layer, which will be collected, but not the intermediate layer, and then precipitated by isopropanol to obtain Total RNA, It can be directly used for Northern hybridization, mRNA purification, in vitro translation, RT-PCR and other molecular biology experiments. If necessary, DNase I can be used to treat Total RNA to remove the trace DNA.
| individual parts making up a compound | 21101 | 21102 |
| AG RNAex Pro Reagent | 100 ml | 200 ml |
Storage temperature: 4 ℃ Protect from light;
Transportation temperature: room temperature.
