ApexHF HS DNA Polymerase Premix - FL

Name: ApexHF HS DNA 聚合酶预混液 - FL
SKU: 870
Availability: InStock

Product Code: AG12207

norm

100 rxns / 50 μl

Clear

- +

¥550

Product Description

This product is ApexHF HS DNA Polymerase FL is a ready-to-use 2x PCR reaction premix. For the PCR reaction, simply add template, primers and water to the premix for amplification. This premix is easy to use, minimizes human error, and provides results in a short period of time. The product is very suitable for the amplification of long fragments, using Human gDNA as template, it can amplify DNA fragments up to 30 kb in length; at the same time, it has the characteristics of high fidelity, high amplification efficiency, high annealing efficiency and fast extension speed. In addition, a monoclonal antibody that can inhibit the activity of DNA polymerase at room temperature is added to this product, which can be used for Hot Start PCR, effectively inhibiting the formation of primer dimers and non-specific amplification.

Product Advantages

① ApexHF HS DNA Polymerase FL is a high-fidelity DNA polymerase with high amplification efficiency, high annealing efficiency and fast extension speed to improve PCR reaction efficiency.
② This product is very suitable for long fragment amplification, and can amplify DNA fragments up to 40kb using λDNA as a template, and up to about 30kb using Human gDNA as a template.
③ Suitable for amplification of DNA fragments with complex structures, such as high GC DNA fragments.

④ A monoclonal antibody that can inhibit the activity of DNA polymerase has been added to this product, which can be used for hot start reaction. Before the PCR reaction, the antibody binds to the enzyme and inhibits the activity of DNA polymerase, effectively inhibiting the formation of primer dimers and non-specific amplification.

⑤ The templates are widely applicable and can be used for efficient PCR reactions using cDNA as a template.

(6) This product is a 2X premix, which can be used for PCR amplification by adding only template, primer and water, which is easy to operate and reduces human error.

Product Composition

individual parts making up a compound	norm
2X ApexHF FL PCR Master Mix*	500 μl x 5 pcs

* Mg in solution²⁺concentration was 2 mM and the concentration of dNTPs was 400 μM .

Preservation and transportation

Storage temperature: -20℃
Transportation temperature: dry ice or -20℃ ice bag transportation

Example 1

Using Human gDNA as a template (lanes 1 to 4), a 30 kb fragment of DNA was amplified; using λDNA as a template (lanes 5 to 8), a 40 kb fragment of DNA was amplified.

The electrophoresis results are shown below:

Example 2

Human gDNA was used as a template to amplify DNA fragments with different GC contents, and both high GC and high AT DNA fragments were well amplified.

The electrophoresis results are shown below:

Example 3

Using Human gDNA as template, the kit can rapidly amplify DNA fragments of different lengths. 4 kb DNA fragments can be amplified with an extension time of 5 sec; 6 kb DNA fragments can be amplified with an extension time of 10 sec.

The electrophoresis results are shown below:

Example 4

Using Human gDNA as template, different template amounts (500 ng, 200 ng, 100 ng, 10 ng, 1 ng, 100 pg) were added to amplify 4 kb DNA fragments, and with template amounts as low as 1 ng, the target fragments could be amplified at a rate of 5 sec/kb.

The electrophoresis results are shown below:

Example 5

Using λDNA as template, different template amounts (5 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg) were added to amplify 4 kb DNA fragments, and the target fragment could be amplified at a rate of 5 sec/kb at template amounts as low as 100 fg.