<i>ChiDigit</i> 探针法芯片式数字 PCR 试剂盒（含 UNG）

ChiDigit Probe-based Chip-based Digital PCR Kit (with UNG)

Product Code: AG12902

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¥1,300

Product Description

This is a 2X Premix Probe DNA Assay Kit for Chip digital PCR (cdPCR). The reaction solution is very simple and quick to prepare, only need to add primers, probes, templates and RNase free water to carry out the PCR reaction, which can effectively reduce the risk of contamination.
This product adopts the superior reaction performance Accurate Taq HS DNA Polymerase with carefully optimized Buffer for efficient and stable PCR amplification in microarrays. This product contains ROX dye for quality control of the effective number of microtiter wells.
The dUTP/UNG Anti-Contamination System has been introduced into this product, which replaces dTTP with dUTP in the PCR reaction, utilizing the fact that UNG enzyme can selectively hydrolyze dU-containing DNA strands without affecting dU-free DNA strands, and removes the contaminating dUTP-containing templates that are introduced into the PCR reaction system, thus preventing the generation of false-positive results in the PCR. The product can effectively prevent the generation of false positive PCR results. At the same time, monoclonal antibody that can inhibit the activity of DNA Polymerase at room temperature is added to the product, which can effectively inhibit non-specific amplification, improve the sensitivity of the reaction and enhance the accuracy of the results.

 

Product Advantages

1. This is a 2X Premix Probe DNA Detection Kit, the preparation of reaction solution is very simple and fast, only need to add primers, probes, templates and RNase free water to carry out the digital PCR reaction, which can effectively reduce the possibility of contamination due to multiple operations.
2. This product adopts hot-start polymerase with superior reaction performance, together with carefully optimized reaction system, featuring high specificity and amplification efficiency.
3. dUTP/UNG is a contamination prevention system that removes contamination of dUTP-containing PCR products, thereby preventing false-positive PCR results and improving the accuracy of the results.

Product Composition
individual parts making up a compound norm
2X Chip dPCR Premix for Probe (UNG Plus)*1 500 μl X 2 pcs
RNase free water 1 ml

*1: contains Accurate Taq HS DNA Polymerase, UNG Enzyme, dN(U)TP Mixture, ROX, and Reaction Buffer.

Preservation and transportation
Storage temperature: -20℃ storage
Transportation temperature: dry ice transportation or -20℃ ice bag transportation

(ABI QuantStudio™ Absolute Q™ Digital PCR System as an example)

Example 1

The product was used to amplify the African swine fever (ASFV) standard (theoretical detection copy number of 4500 copies), and the experimental results were as follows:

The results are shown above:

1, QC QC effective holes for 20479, to meet the quality control requirements (quality control requirements for the total number of effective microporous greater than 20000);
2. Positive micropores are evenly dispersed and no positive micropores are aggregated;
3. The yin and yang partitions are clear, which helps in the setting of the threshold line;
4. The actual amount of templates detected was 4296 copies, which is consistent with the theoretical number of copies.

Example 2

The product was used to amplify Human TFR gene, the theoretical amount of DNA template added were 100 copies, 10 copies, 1 copy, 0 copy (negative control), the experimental results are as follows:

The results are shown above:
1, QC QC effective holes for 20448, to meet the quality control requirements (quality control requirements for the total number of effective microporous greater than 20000);
2. Positive micropores are evenly dispersed and no positive micropores are aggregated;
3. The yin and yang partitions are clear, which helps in the setting of the threshold line;
4. The actual amount of templates detected were 90 copies, 8 copies, 2 copies and 0 copies, which were in line with the theoretical number of copies.

Example 3

The Human CEPH gene was amplified by this product for copy number variation analysis, and a four-channel assay was performed, with the FAM, VIC, ABY and JUN channels detecting one locus each (the theoretical ratio of detected copy number of each channel, FAM : VIC : ABY : JUN = 1:1:1:2), and the results were as follows:

The results are shown above:
1, QC QC effective holes for 20461, to meet the quality control requirements (QC requirements for the total number of effective microporous greater than 20000);
2. Positive micropores are evenly dispersed and no positive micropores are aggregated;
3. The yin and yang partitions are clear, which helps in the setting of the threshold line;
4. Quadruple testing is available;
5. The number of copies detected by different fluorescence channels meets FAM : VIC : ABY : JUN ≈ 1:1:1:2, which is consistent with the theoretical copy number.

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