This product is available from OD600 ≤ 600 OD(例如:菌液 OD 值为 2.0 时,菌液体积为 300 ml)的培养菌液中提取获得多至 1 mg 无内毒素质粒 DNA。本产品采用优化的碱裂解法,使用硅胶膜吸附技术结合独特的内毒素清除系统和漂洗系统,可在提取质粒的同时有效去除内毒素、蛋白质等杂质。纯化获得的质粒 DNA 收量高,内毒素含量低,可直接用于转染、体外转录翻译、酶修饰等分子生物学实验。
| individual parts making up a compound | norm |
| RNase A (10 mg/ml) | 1.2 ml |
| Buffer PRS-2*2 | 120 ml |
| Buffer PLS-2 | 100 ml |
| Buffer PBS-2 | 100 ml |
| Buffer ER | 30 ml |
| Buffer PWA | 80 ml |
| Buffer PWB-2*3 | 75 ml |
| Endo-free Elution Buffer | 20 ml |
| Plasmid DNA Ultra Columns | 10 sets |
| RNase Free Tubes | 10 pcs |
| Column Filter | 10 pcs |
*1: RNase A (10 mg/ml) is packaged in Package 2-1 and should be stored at -20℃; the rest of the components are packaged in Package 2-2 and should be stored at room temperature (15 ~ 30℃).
*2: Before the first use of Buffer PRS-2, it is necessary to add RNase A into Buffer PRS-2 (the volume ratio of RNase A to Buffer PRS-2 is 1:100), mix it well, and then mark the bottle. Buffer PRS-2 with RNase A should be stored at 2~8℃ for 6 months.
*3:Buffer PWB-2 在首次使用前,请添加 175 ml 的无水乙醇(Buffer PWB-2 与无水乙醇体积比为 3∶7 ),混合均匀后在瓶子上做好标记,室温下保存。
Preservation Temperature:
Package 2-1 -20°C Storage
Package 2-2 Storage at room temperature (15 ~ 30°C)
Transportation temperature:
Package 2-1 Dry Ice or -20°C Ice Pack Transportation
Package 2-2 Room Temperature Transportation





