SteadyPure Endotoxin-free plasmid ultra-high volume extraction kit (column membrane method)

Product Code: AG21043

- +

¥1,600

Product Description

This product is available from OD600 ≤ 600 OD(例如:菌液 OD 值为 2.0 时,菌液体积为 300 ml)的培养菌液中提取获得多至 1 mg 无内毒素质粒 DNA。本产品采用优化的碱裂解法,使用硅胶膜吸附技术结合独特的内毒素清除系统和漂洗系统,可在提取质粒的同时有效去除内毒素、蛋白质等杂质。纯化获得的质粒 DNA 收量高,内毒素含量低,可直接用于转染、体外转录翻译、酶修饰等分子生物学实验。

Product Composition*1
individual parts making up a compound norm
RNase A (10 mg/ml) 1.2 ml
Buffer PRS-2*2 120 ml
Buffer PLS-2 100 ml
Buffer PBS-2 100 ml
Buffer ER 30 ml
Buffer PWA 80 ml
Buffer PWB-2*3 75 ml
Endo-free Elution Buffer 20 ml
Plasmid DNA Ultra Columns 10 sets
RNase Free Tubes 10 pcs
Column Filter 10 pcs

*1: RNase A (10 mg/ml) is packaged in Package 2-1 and should be stored at -20℃; the rest of the components are packaged in Package 2-2 and should be stored at room temperature (15 ~ 30℃).
*2: Before the first use of Buffer PRS-2, it is necessary to add RNase A into Buffer PRS-2 (the volume ratio of RNase A to Buffer PRS-2 is 1:100), mix it well, and then mark the bottle. Buffer PRS-2 with RNase A should be stored at 2~8℃ for 6 months.
*3:Buffer PWB-2 在首次使用前,请添加 175 ml 的无水乙醇(Buffer PWB-2 与无水乙醇体积比为 3∶7 ),混合均匀后在瓶子上做好标记,室温下保存。

Preservation and transportation

Preservation Temperature:
Package 2-1 -20°C Storage
Package 2-2 Storage at room temperature (15 ~ 30°C)
Transportation temperature:
Package 2-1 Dry Ice or -20°C Ice Pack Transportation
Package 2-2 Room Temperature Transportation