<i>Evo M-MLV</i> 一步法 RT-qPCR 试剂盒 III（探针法）

Evo M-MLV One-step RT-qPCR kit III (probe method)

Product Code: AG11715

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¥1,800

Product Description

This product is specialized for One Step RT-qPCR using probe method. Reverse transcription and qPCR reaction are completed in the same tube, which is simple and fast, and can effectively reduce the risk of contamination. The reverse transcriptase and buffer of this product have been optimized for a wide range of applications, especially for the amplification of RNAs with high GC content or complex secondary structures. The product utilizes the strong extension ability of Evo M-MLV II Reverse transcriptase, integration of hot start Pro Taq With the superior performance of HS, cDNA can be efficiently synthesized and efficiently and stably amplified by qPCR in a short period of time, which is very suitable for the detection of trace RNA such as viral RNA.

Product Advantages

① This product is a one-step RT-qPCR reaction kit, completing the reverse transcription and qPCR reaction in a single tube, simplifying the operation, improving efficiency, and effectively reducing the possibility of contamination from multiple operations.
②The product is made of a material with high elongation capacity. Evo M-MLV II Reverse transcriptase, high performance hot start enzyme Pro Taq HS and optimized reaction system with high specificity and amplification efficiency.
③This product can rapidly and accurately detect trace amounts of RNA.
④ Suitable for amplification of RNA with high GC content or complex secondary structure.
⑤ This product can be used in multiprobe assays.

Product Composition
individual parts making up a compound norm
2X One Step RT-qPCR Buffer III (Probe)*1 1.25 ml x 2 pc
Pro Taq HS DNA Polymerase (5 U/μl) 100 μl
Evo M-MLV RTase Enzyme Mix III*2 100 μl
RNase free water 1 ml x 3 pc

*1: Contains dNTP Mixture with reaction buffer.
* :: 2: Contains Evo M-MLV RTase, RNase Inhibitor.

Preservation and transportation
Storage temperature: -20℃
Transportation temperature: dry ice or -20℃ ice bag
Example 1

Human Total RNA (200 ng~200 fg) was used as a template for One Step RT-qPCR to detect TFRC Gene (GC content 38%) using this kit.

The results are shown above:
① The amplification efficiency was 91.99%, R2= 0.9986;
② Accurate quantification can be performed over a wide template range, and the amplification curves of RT-qPCR reactions with 200 ng~200 fg Total RNA show good linearity.

Example 2

Human Total RNA (200 ng~200 fg) was used as a template for One Step RT-qPCR using this kit. β Actin Gene (GC content 58.7%).

The results are shown above:
① The amplification efficiency was 90.34%, R2= 0.9988;
② It can be accurately quantified over a wide template range, and the amplification curves of RT-qPCR reactions with 200 ng~200 fg Total RNA show good linearity.

Example 3

Pork Total RNA (100 ng~10 pg) was used as a template for One Step RT-qPCR to detect IGF2 Gene (GC content 75.2%) using this kit.

The results are shown above:
① The amplification efficiency was 94.06%, R2= 0.9998;
② Accurate quantification can be performed over a wide template range, and the amplification curves of RT-qPCR reactions with 100 ng~10 pg Total RNA show good linearity.

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