This product is a specialized kit for One Step RT-qPCR using SYBR Green I chimeric fluorescence method. Reverse transcription and qPCR reactions are completed in the same tube, which is simple and fast to operate and can effectively reduce the risk of contamination. The product utilizes a high elongation capacity Evo M-MLV Reverse transcriptase, integrated hot start Pro Taq With the superior performance of HS, it is widely applicable to the amplification of various templates, and can be used to efficiently synthesize cDNA and efficiently and stably amplify qPCR in a short period of time.
① This is a one-step RT-qPCR reaction kit, which completes the reverse transcription and qPCR reaction in a single tube, simplifying the operation, improving the efficiency, and effectively reducing the possibility of contamination by multiple operations.
②This product adopts a strong extension ability Evo M-MLV Reverse transcriptase, high performance hot start enzyme Pro Taq HS and optimized reaction system with high specificity and amplification efficiency.
③ This product can detect trace amounts of RNA quickly and accurately.
| individual parts making up a compound | norm |
| 2X One Step RT-qPCR Buffer II (SYBR)*1 | 1.25 ml x 2 pcs |
| One Step Enzyme Mix II *2 | 200 μl |
| RNase free water | 1 ml x 3 pcs |
*1: This solution contains dNTP Mixture, Reaction Buffer, and SYBR dye, store away from light.
* :: 2: Contains Evo M-MLV RTase, RNase Inhibitor.Pro Taq HS DNA Polymerase.
Transportation temperature: dry ice transportation or -20℃ ice bag transportation.
