<i>Evo M-MLV</i> 反转录试剂预混液（用于 qPCR ）

Evo M-MLV Reverse transcription reagent premix (for qPCR)

Product Code: AG11706

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¥1,400

Product Description

This product is a premix of reverse transcription reagents for Real Time RT-PCR. It utilizes a high elongation capacity Evo M-MLV Reverse transcriptase can efficiently synthesize cDNA in 15 min, and the synthesized cDNA can be used for chimeric and probe-based qPCR analysis.5X Evo M-MLV RT Master Mix contains all the necessary components for the reverse transcription reaction, only need to add RNA template and water to directly carry out the reaction, easy to use, can reduce the loss of reagents and experimental errors.
Optimized for Oligo dT (18T) Primer and Random 6 mers Primer dosage, this product makes reverse transcription products compatible with both chimeric and probe-based qPCR analyses for efficient gene expression analysis.

Product Advantages

① It can synthesize cDNA quickly and efficiently, and can be used for chimeric and probe-based qPCR analysis in only 15 min.
② This product is a premixed Premix, which can be directly reacted by adding RNA template and water.
③This product is optimized for Oligo dT (18T) Primer and Random 6 mers Primer dosage so that the reverse transcription product is compatible with chimeric and probe-based qPCR analysis.

Product Composition
individual parts making up a compound norm
5X Evo M-MLV RT Master Mix *1 400 μl
RNase free water 1 ml X 2 pcs

*1: containsEvo M-MLV RTase, RNase Inhibitor, dNTP, Oligo dT (18T) Primer, Random 6 mers Primer, Reaction Buffer.

Preservation and transportation
Storage temperature: -20℃ storage
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
Example 1

This product was used to reverse transcribe mouse total RNA, and the starting amount of RNA was 1 μg~1 pg. 2 μl of cDNA stock solution was taken from this cDNA as a template, and the mouse total RNA was detected by chimeric qPCR. GAPDH Gene (This example of qPCR was performed using our product SYBR Green. Pro Taq (HS Premixed qPCR Kit, Code No. AG11701 was performed).

The results are shown above:
① The amplification efficiency was 108.31 TP3T and R2= 0.992.
② This product has a high reversal efficiency and can be accurately quantified over a wide template range. The cDNA amplification curves obtained by reverse transcription of 1 μg ~ 1 pg Total RNA show good linearity.
③ The melting curve has a single peak type and good amplification specificity.

Example 2

Total RNA from 293T cells was reverse transcribed using this product, and the starting amount of RNA was 1 μg~10 pg. 2 μl of cDNA stock solution was taken from this cDNA as a template, and Human RNA was detected by chimeric qPCR. TFRC Gene (SYBR Green was used for qPCR in this experiment) Pro Taq (HS Premixed qPCR Kit, Code No. AG11701 was performed).

The results are shown above:
① Amplification efficiency was 97.8%, R2= 0.997.
② This product has a high reversal efficiency and can be accurately quantified over a wide template range. The cDNA amplification curves obtained by reverse transcription of 1 μg ~ 10 pg Total RNA show good linearity.
③ The melting curve has a single peak type and good amplification specificity.

Example 3

The total RNA of 293T cells was reverse transcribed using this product, and the starting amount of RNA was 1 μg ~ 1 pg. Then 2 μl of cDNA stock solution was taken from this cDNA as a template, and the Human RNA was detected by qPCR using the probe method.β-actin Genes (Our products were used for qPCR in this experiment) Pro Taq (HS Pre-mixed Probe-based qPCR Kit, Code No. AG11704 was performed).

The results are shown above:
① The amplification efficiency was 93.1%, R2= 0.999.
② This product has a high reversal efficiency and can be accurately quantified over a wide template range. The cDNA amplification curves obtained by reverse transcription of 1 μg ~ 1 pg Total RNA show good linearity.

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