<i>Evo Super M-MLV</i> 一步法RT-PCR 预混液

Evo Super M-MLV One-step RT-PCR premixes

Product Code: AG11624

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¥1,177

Product Description

This product is a one-step RT-PCR kit using RNA as template. Reverse transcription and PCR reactions are completed continuously in the same reaction system, which is easy and fast to operate, and there is no need to add any reagent during the reaction process, which can effectively reduce the risk of contamination.
This product has been optimized to realize the one-tube operation of reverse transcriptase, amplification enzyme and reaction buffer, which is easy and convenient to operate. The product integrates the superior reaction performance of Evo Super M-MLV Reverse transcriptase,Pro Taq HS DNA polymerase and auxiliary proteins, and optimize the buffer composition in the product, which makes the product has the characteristics of wide applicability to different templates, high amplification efficiency, high amplification yield, high detection sensitivity, etc., and is more convenient for the amplification analysis of RNA samples.

Product Advantages

1. Reverse transcriptase, amplification enzyme and reaction buffer have been mixed into one tube in this product, and amplification can be carried out by adding primers and templates, which is easy and convenient to operate.
2. This product is a one-step RT-PCR reaction kit, which can complete the reverse transcription and PCR reactions in a single tube, simplifying the operation, improving the efficiency, and effectively reducing the possibility of contamination caused by multiple operations.
3. This product is characterized by wide applicability to different templates, high yield, strong amplification specificity and high detection sensitivity, which is more convenient for amplification analysis of RNA samples.

Product Composition
individual parts making up a compound norm
2X One Step RT-PCR Mix 1.25 ml
RNase free water 1 ml x 2 pcs

 

Preservation and transportation
Storage temperature: -20℃ storage
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
Example 1

One-step RT-PCR amplification of fragments of different lengths (129 bp-GC 38.0%, 319 bp-GC 53.7%, 780 bp-GC 63.7%, 1.5 kb- GC 43.0%, 3.4 kb-GC 48.8%, 3.8 kb-GC 38.9%, 5 kb-GC 39.5%, 7.5 kb-GC 42.8%, 8 kb-GC 42.9%, 13 kb-GC 50.9%).

The electrophoresis results are as follows:

Example 2

One-step RT-PCR amplification of 537 bp fragments using 293T cell Total RNA as template. The amount of template added is 1 μg, 100 ng, 10 ng, 1 ng, 100 pg, 10 pg, 1 pg, and the amount of template detected can be up to 1 pg.

The electrophoresis results are as follows:

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