<i>Evo Super M-MLV</i> 反转录聚合酶 III

Evo Super M-MLV Reverse transcription polymerase III

Product Code: CM0267

- +

¥3,000

Product Description

Evo Super M-MLV Reverse transcription polymerase III is a reverse transcriptase mutant genetically modified on the basis of M-MLV (Moloney murine leukemia virus) and obtained by purification through an E. coli recombinant expression system.
Evo Super M-MLV Reverse transcription polymerase III has high thermal stability and a wide range of reaction temperatures (42℃ ~ 65℃), as well as strong anti-interference ability, no RNase H activity, high synthesis efficiency, fast reaction speed and high sensitivity.
Evo Super M-MLV Reverse transcription polymerase III can efficiently synthesize 1st Strand cDNA at 42℃~65℃, and its optimal reaction temperature is 55℃, which can also reverse complex templates well. It is also suitable for the construction of full-length cDNA libraries.

 

Product Advantages

1. Strong thermal stability: the reverse transcription reaction can be carried out under the conditions of 42℃~65℃, and its optimal temperature is 55℃, which is favorable for the synthesis of RNA templates with complex secondary structure.
2. Strong resistance to inhibition: good resistance to inhibitors remaining in the RNA (e.g. guanidine isothiocyanate, ethanol, EDTA and heme, etc.).
3. Wide range of applications: suitable for cDNA synthesis and full-length cDNA library construction.
4. Highly transcriptional: can synthesize fragments up to 13 kb in length.
5. Fast transcription: reverse transcription reaction can be completed within 5 min at the earliest.

Product Composition
individual parts making up a compound norm
Evo Super M-MLV RTase III ( 200 U/μl ) 100 μl
5X RTase Reaction Buffer* 800 μl

*:5X RTase Reaction Buffer composition: 250 mM Tris-HCl ( pH8.3 at 25℃) , 375 mM KCl , 45 mM MgCl2

Preservation and transportation
Storage temperature: -20℃ storage
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
Example 1

Total RNA from 293T cells and mouse heart RNA were used as templates and were inverted using this product (reaction temperature was 55°C), followed by 2X L-Exp Taq Master Mix (Code No. AG11417) amplified fragments of different lengths, and good amplification results were obtained.

Example 2

Different fragments (2.6 kb and 4 kb) were amplified after inversion at different temperatures (42°C, 50°C, 55°C, 60°C, 65°C) using 293T cell Total RNA as template (template amount of 1 μg and 100 ng) with the product:Evo Super M-MLV RTase Ⅲ has good inversion performance in the temperature range of 42℃~65℃, and the recommended optimal inversion temperature is 55℃. The electrophoresis results are shown below:

Example 3

293T Total RNA (100 ng ~ 1 pg) was used as template, RNase free water was used as negative control instead of template, and the product was reversed using this product, and the reversed product was paired with 2X Premix Probe qPCR Kit for ASFV (UNG Plus, Primer Free) (Code No. AG11731) for quantitative detection (RPP30 gene, GC content of 62%, CY5 channel). AG11731) for quantification (RPP30 gene, GC content 62%, CY5 channel). The quantification instrument used was an ABI QuantStudioTM 5 Real-Time PCR Systems. the results of the experiments were as follows:

The results are shown above:
1, different concentrations of templates can get good amplification within 45 cycles.
2. Negative controls were not detected at 45 cycles.
3, the amplification efficiency was 96.36%, R2= 0.9994.

Related products