After drinking alcohol, ethanol is oxidized to acetaldehyde in the liver by the enzyme ethanol dehydrogenase (ADH), and acetaldehyde is further converted to harmless acetic acid as a substrate catalyzed by the enzyme acetaldehyde dehydrogenase 2 (ALDH2); ALDH2 is a key enzyme for alcohol metabolism in the human body, and a mutation in the ALDH2 gene has a common single-nucleotide polymorphism (SNP) site (rs671), which reduces the body's ability to detoxify acetaldehyde. Mutations in the ALDH2 gene reduce the body's ability to detoxify acetaldehyde and may increase the risk of diseases such as cancer. Therefore, SNP testing of the ALDH2 gene can be used to analyze the ability to metabolize alcohol by examining the type of ALDH2 mutation (wild-type, heterozygous, or mutant), and to guide individualized alcohol consumption to prevent disease.
This product is based on TaqMan MGB method and specially developed for ALDH2 gene, the 12.5X GenDiff SNP Primer & Probe Mix for ALDH2 Genotyping in the kit contains two different fluorescent probes, FAM and VIC, FAM fluorescent channel is used for detecting wild type (G type), and VIC fluorescent channel is used for detecting mutant (A type), which can be used with 2X GenDiff SNP qPCR Mix for ALDH2 Genotyping and Lysis Buffer in the kit. The FAM fluorescence channel is used for detecting wild type (G type) and the VIC fluorescence channel is used for detecting mutant type (A type), and it can be used with the 2X GenDiff SNP qPCR Mix for ALDH2 Genotyping and the Lysis Buffer in the kit; this product is easy to operate, and you can observe the presence or absence of the two fluorescence probes after you have scraped the oral epithelium and lysed them into Lysis Buffer for quantitative amplification. The genotyping results can be obtained by observing the presence or absence of two types of fluorescent probes. The product is characterized by easy operation, high sensitivity, high reproducibility and strong anti-interference.
① The product is simple to operate, oral epithelial cells can be sampled directly after quantitative amplification, by observing the presence or absence of the two fluorescent signals can be determined by the results, with the advantages of simple operation, simple analysis and reliable results.
② This product is characterized by high sensitivity, high differentiation, high repeatability and strong anti-interference.
| individual parts making up a compound | norm |
| 2X GenDiff SNP qPCR Mix for ALDH2 Genotyping | 625 μl |
| 12.5X GenDiff SNP Primer & Probe Mix for ALDH2 Genotyping*1 | 100 μl |
| Lysis Buffer*2 | 1 ml x 5 pcs |
| RNase free water | 1 ml |
*1: This reagent contains primer probes to be stored out of the light, of which FAM is labeled as G-type and VIC as A-type.
*2: This reagent is a lysate and can be stored at room temperature after opening.
Transportation temperature:Dry ice transportation or -20°C ice pack transportation.
