<i>GenDiff</i> SNP 预混型探针法 qPCR 试剂盒（ARMS）

GenDiff SNP Premixed Probe-based qPCR Kit (ARMS)

Product Code: AG12803

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¥1,600

Product Description

This kit is a combination of ARMS-PCR and Taqman probe for SNP genotyping. It is a 2X pre-mixed reagent that requires only the addition of primer probe, template and RNase free water, making the preparation of the reaction solution very simple. The PCR reaction system is optimized with the use of a high performance Accurate Taq HS DNA Polymerase can amplify templates of different complexity with high amplification efficiency and sensitivity. Meanwhile, a monoclonal antibody that can inhibit the activity of DNA polymerase at room temperature is also added, which can effectively inhibit the formation of primer dimers and non-specific amplification, and can carry out highly sensitive Real Time PCR reactions, which is very suitable for SNP genotyping. It can perform highly sensitive Real Time PCR reaction, which is very suitable for SNP genotyping.

 

Product Advantages

1. It is a 2X premixed solution, easy to prepare, only need to add primer probe, template and RNase free water to carry out qPCR reaction.
2. The results of this product are easy to analyze. Genotyping can be performed by analyzing the CT difference between two genotypes, which is simple to analyze and the results are reliable.
3. This product has optimized the PCR reaction system with high sensitivity, excellent typing performance and good versatility.

Product Composition
individual parts making up a compound norm
2X Probe qPCR Mix (ARMS)*1 1 ml X 5 pcs

*1: A white precipitate may form when the solution is stored at -20°C. Dissolve on ice or by hand before use and mix upside down until the precipitate disappears. Do not vortex.

Preservation and transportation
Storage temperature: -20℃ storage
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
Example 1

Use this product for fluorescence quantitative PCR in mice. GAPDH genes, the template was added at 100 ng - 1 pg, and cDNA was synthesized using the company's Evo M-MLV Reverse Transcription Premix Kit (with gDNA removal reagent for qPCR) Ver.2 (Code No. AG11728). The quantification instruments used were ABI QuantStudioTM 5 Real-Time PCR Systems. the results are shown below:

The results are shown above:
1. Working curve R2= 0.996, amplification efficiency 99%;
2. Accurate quantification is possible over a wide template range, with good linearity in the amplification curve over the 100 ng - 1 pg concentration range.

Example 2

ABI QuantStudio is used for the quantification of different genotypes (wild type, mutant) of different samples.TM 5 Real-Time PCR Systems, different types of templates can be accurately differentiated by comparing the differences in Ct values, and the results are as follows:

The results are shown above:
1. In the typing result 1, according to the amplification curve, it was found that the wild-type template amplified normally, and the mutant template did not have obvious peaks, which could distinguish the mutant type from the wild-type significantly;
2. In typing result 2, the difference between mutant and wild-type Ct was found to be ≥ 14 according to the amplification curve, which can significantly distinguish the mutant from the wild-type.

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