This is a kit that utilizes T7 RNA Polymerase for in vitro transcription to obtain large amounts of RNA in a short period of time. T7 RNA Polymerase synthesizes RNA downstream of the T7 promoter sequence using a template such as linearized plasmid DNA with the T7 promoter, PCR products or chemically synthesized DNA fragments (T7 promoter sequence “TAATACGACTCACTATA”), and NTP as the substrate. The T7 RNA Polymerase synthesizes RNA downstream of the T7 promoter, using NTP as the substrate, and synthesizes 100 to 200 μg of RNA per system, while transcribing fragments up to 10 kb in length. The transcribed RNA product can be used for RNA structure and function, RNAase protection, probe hybridization, RNAi, microinjection, in vitro translation and mRNA vaccines.
1. The substrate and enzyme in this product are presented in the form of premix, which reduces the experimental steps and is simple and convenient.
2. This product has a high RNA yield for transcription, each reaction system can obtain 100 ~ 200 μg of high quality RNA.
| ingredient | norm |
|---|---|
| 10X In Vitro Transcription Buffer | 100 μl |
| NTP Mix (25 mM each) | 400 μl |
| T7 RNA Polymerase Mix | 100 μl |
| RNase free water | 1 ml |
Storage temperature: -20℃ storage
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
