This product is made of our superior performance Accurate Taq The PCR reaction system was carefully optimized by adding high-fidelity enzyme with partial 3′-5′ Exonuclease activity (Proof reading activity). The PCR reaction system not only has a very strong performance in amplifying long fragments, but also has the ability to amplify complex templates, and it can amplify DNA fragments up to ~24 kb by using the Human genome DNA as a template, and it has a high success rate for difficult to amplify high GC content templates (~75%). Moreover, the amplification rate of high GC content template (~75%), which is difficult to amplify, is high.
at the same time L-Exp Taq The HS DNA Polymerase also contains a blend of Taq monoclonal antibodies for Hot Start PCR, which bind to and inhibit the Taq enzyme prior to the start of the reaction, thus inhibiting non-specific amplification caused by non-specific annealing of the primers at low temperatures. Once the PCR reaction is initiated, the antibody is inactivated during the initial DNA denaturation step and can be used under routine PCR reaction conditions. Most PCR products obtained with this product have an A base at the 3’ end and can be cloned directly into T vectors.
② L-Exp Taq HS DNA Polymerase adds high-fidelity enzymes relative to the Accurate Taq Has higher fidelity and stronger amplification ability. High amplification efficiency and low mismatch rate under normal PCR amplification conditions.
| individual parts making up a compound | norm |
| L-Exp Taq HS DNA Polymerase (5 U/μl) | 25 μl |
| 5X L-Exp Taq PCR Buffer (Mg)2+ plus) | 500 μl |
| dNTP Mix (10 mM each) | 100 μl |
Transportation temperature: dry ice or -20℃ ice bag transportation
