<i>Pro Taq</i> HS 预混型探针法 qPCR 试剂盒 III

Pro Taq HS Premixed Probe qPCR Kit III

Product No.:AG11730

- +

¥1,200

Product Description

This product is a special reagent for qPCR using the probe method. It is a 2X Premix reagent, which is very simple to prepare and widely applicable, and can be used for amplification of various templates. The PCR reaction system of this product has been optimized, adopting the superior reaction performance of the Pro Taq HS system (mixed with Taq antibody) can effectively inhibit non-specific amplification, improve PCR amplification efficiency, can perform Real Time PCR reaction with high sensitivity, and can obtain a good standard curve in a wide quantitative region, so as to accurately quantify and detect target genes.

Product Advantages

① This product is a 2X premix, the reaction solution is very simple to prepare, only need to add primers, templates, probes and RNase free water can be qPCR reaction.
②This product adopts the superior performance of the Pro Taq HS and optimized reaction system with high specificity and amplification efficiency.

Product Composition
individual parts making up a compound norm
2X Pro Taq HS Probe Premix III 1 ml x 5 pc
Preservation and transportation
Storage temperature: -20℃
Transportation temperature: dry ice or -20℃ ice pack transportation
Example 1

This kit was used for the detection of Human by fluorescent probe qPCR method. TFRC Gene (GC content 38.0%), and the amount of template cDNA added (equivalent to the amount of Total RNA) was 100 ng ~ 1 pg. cDNA was synthesized using our own cDNA synthesizer. Evo M-MLV Reverse Transcription Kit II (with gDNA removal reagent for qPCR) (Code.AG11711). Quantification instrument used: ABI QuantStudioTM 5 Real-Time PCR Systems.

The results are shown above:
① The amplification efficiency was 100.61% and R2= 0.9968;
② Accurate quantification can be performed over a wide template range, and the amplification curve shows good linearity over the range of 100 ng ~ 1 pg cDNA (equivalent to Total RNA amount).

Example 2

This kit was used for the detection of Human by fluorescent probe qPCR method. β-Actin The amount of template cDNA added (equivalent to the amount of Total RNA) was 100 ng ~ 100 fg. cDNA was synthesized using our own cDNA synthesizer. Evo M-MLV Reverse Transcription Kit II (contains gDNA removal reagents for qPCR) (Code.AG11711). Quantification instrument used: ABI QuantStudioTM 5 Real-Time PCR Systems.

The results are shown above:
① The amplification efficiency was 93.57%, R2= 0.9995;
② Accurate quantification can be performed over a wide template range, and the amplification curve shows good linearity in the range of 100 ng ~ 100 fg cDNA (equivalent to Total RNA amount).

Example 3

This kit was used for the detection of Pig by the fluorescent probe qPCR method. TGFB1 The cDNA was synthesized using the cDNA from our company (GC content 70.9%), and the amount of template cDNA added (equivalent to the amount of Total RNA) was 10 ng ~ 1 pg. cDNA was synthesized using the cDNA from our company. Evo M-MLV Reverse Transcription Kit II (with gDNA removal reagent for qPCR) (Code.AG11711). Quantification instrument used: ABI QuantStudioTM 5 Real-Time PCR Systems.

The results are shown above:
① The amplification efficiency was 92.23%, R2= 0.9990;
② Accurate quantification can be performed over a wide template range, and the amplification curve shows good linearity over the concentration range of 10 ng ~ 1 pg cDNA (equivalent to Total RNA amount).

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