SteadyPure Plant Genome DNA Extraction Kit Ver.2

Item No.: AG21011 / AG21012

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Price range: ¥400 through ¥1,800

Product Description

SteadyPure Plant Genomic DNA Extraction Kit Ver.2 is a product for rapid and efficient extraction of genomic DNA from simple and complex plants. The product adopts optimized lysis system, which has strong lysis ability for plant samples. During the extraction process, there is no need to use toxic phenol-chloroform extraction or time-consuming alcohol precipitation, and it can effectively remove impurities such as proteins and salts. The extracted genomic DNA is of good integrity, high purity, stable and reliable quality. The genomic DNA is dissolved in Elution Buffer or sterilized water, and can be used directly for PCR amplification, qPCR amplification, library construction, gDNA sequence analysis, etc.

Product Composition
individual parts making up a compound AG21011 (50 rxns)
RNase A (10 mg/ml)*1 500 μl
50 X DTT Buffer*1 700 μl
Buffer LS-3 Ver.2 25 ml
Buffer LS-4 Ver.2 25 ml
Buffer PA 1.6 ml X 2 pcs
Buffer BS-2 Ver.2*2 12 ml
Buffer WA 25 ml
Buffer WB*3 27 ml
Elution Buffer 10 ml
Plant DNA Mini Columns 50 sets
Collection tubes 50 pcs

*1 These two components are packaged in Package 2-1 and need to be stored at -20℃; the rest of the components are packaged in Package 2-2 and stored at room temperature (15 ~ 30℃).
*2 Before using Buffer BS-2 Ver.2 for the first time, add 18 ml of anhydrous ethanol (Buffer BS-2 Ver.2 to anhydrous ethanol volume ratio 2:3), mix well, and mark the bottle.
*3 Before using Buffer WB for the first time, please add 63 ml of anhydrous ethanol (Buffer WB to anhydrous ethanol volume ratio of 3:7), mix well and mark the bottle.

 

Preservation and transportation

Preservation Temperature:
Package 2-1 is stored at -20°C;
Package 2-2 Room Temperature (15 ~ 30°C)

Transportation temperature:
Package 2-1 is shipped on dry ice or in -20°C ice packs;
Package 2-2 is shipped at room temperature.

sample extraction sample size (of a liquid or gas) Lysate LS-3 Lysis Solution LS-4
Rice Leaf 100mg
tomato leaf 100mg /
bamboo leaves 100mg
spinach leaf 100mg /
cigar 100mg /
Sweet Potato Leaf 100mg /
Banana pulp 100mg /
Moonflower petal 100mg /
pine needles 100mg /
aloe vera leaf 100mg /
Potato cubes Stems 100mg /
orange peel 100mg /
Sweet potato tuber Stem 100mg /
Grape Leaf Slice 100mg /
Soybean leaves 100mg /
wheat 100mg /
rapeseed 100mg /

Description:
1. √ indicates that extraction was performed with this lysate, / indicates that extraction was not performed with this lysate.
2. 100 mg of sample volume is the recommended amount, if the amount of DNA collected is too low, you can increase the sample volume to 200 mg, and at the same time increase the amount of lysate by the same volume.