This product removes paraffin wax in an environmentally friendly way and is safe and non-toxic. Total RNA (including mRNA and Small RNA) can be extracted from 3~10 paraffin sections of 5~10 μm thickness, paraffin blocks of less than 30 mg, and formalin-fixed tissue samples of less than 30 mg. An optimized lysis system is used to release Total RNA from paraffin samples, overcoming the inhibitory effect caused by formalin cross-linking. Total RNA extracted with this product is of high purity and stable quality, and can be used for downstream experiments such as RT-qPCR and NGS.
| individual parts making up a compound | norm |
| Proteinase K (20 mg/ml) | 1 ml |
| DNase I (RNase free) | 200 μl |
| 10X DNase I Buffer | 1 ml |
| Buffer DP | 30 ml |
| Buffer LS-2 | 13 ml |
| Buffer BS-4 | 11 ml |
| Buffer RWA*2 | 14 ml |
| Buffer RWB*2 | 27 ml |
| RNase Free Water*3 | 10 ml |
| FFPF RNA Mini Columns | 50 sets |
| Collection tubes | 50 pcs |
| RNase Free Tubes*4 | 50 pcs |
*1: Proteinase K (20 mg / ml), DNase I (RNase free) and 10X DNase I Buffer are packaged in Package 2-1 and should be stored at -20℃; the rest of the components are packaged in Package 2-2 and should be stored at room temperature (15 ~ 30℃).
*2: Before the first use of Buffer RWA, please add 21 ml of anhydrous ethanol (the volume ratio of Buffer RWA to anhydrous ethanol is 2:3); before the first use of Buffer RWB, please add 63 ml of anhydrous ethanol (the volume ratio of Buffer RWB to anhydrous ethanol is 3:7). After mixing well, mark the bottle and store at room temperature.
*3: RNase Free Water is recommended to be stored at -20°C after opening.
*4: RNase Free Tubes are for elution of RNA only. Centrifuge tubes for pre-lysis should be provided.
Preservation Temperature:
Package 2-1 -20°C Storage
Package 2-2 Room Temperature (15-30°C) Storage
Transportation temperature:
Package 2-1 -20℃ Ice bag transportation or dry ice transportation
Package 2-2 Room Temperature Transportation
