This product can rapidly extract total RNA from ≤1.0E+07 cultured cells (HL60, 293T, etc.) and ≤10 mg of tissue samples (heart, liver, kidney, brain, stomach, etc.) The kit adopts a unique lysis system, which eliminates the need for phenol-chloroform extraction, and it can inhibit the action of nuclease while lysing the cells or tissues rapidly to maintain the integrity of the RNA, and it has the advantages of simple and rapid operation, good purity and high yield. It has the advantages of simple and fast operation, good purity and high yield. The purified Total RNA can be directly used in RT-qPCR, RT-PCR, Northern hybridization, mRNA purification, in vitro translation and other molecular biology experiments.
| individual parts making up a compound | norm |
| Buffer QLS | 30 ml |
| Buffer QWB*1 | 15 ml |
| RNase Free Water*2 | 10 ml |
| Quick RNA Mini Columns | 50 sets |
| Collection tubes | 50 pcs |
| RNase Free Tubes*3 | 50 pcs |
*1 Before using Buffer QWB for the first time, please add 35 ml of 100% ethanol (Buffer QWB to anhydrous ethanol volume ratio of 3:7), mix well, mark the bottle and store at room temperature.
*2 Recommended storage at -20°C after opening.
*3 This component is for elution of RNA only; centrifuge tubes for pre-lysis should be provided.
Storage temperature: room temperature (15 - 25℃).
Transportation temperature: room temperature.
