This product is suitable for use with tissue samples (≤5 mg tissue samples such as micro laser cut samples, puncture samples, etc.) and cellular samples (≤5 x 10) from trace amounts.5 The RNA is extracted from cells as small as 10 cells (e.g., flow-sorted cells) using a unique lysate that enhances selective binding of RNA to the in silico plasma membrane and inactivates RNA catabolizing enzymes, and a Carrier RNA is added to protect the RNA from degradation during the extraction process. The gDNA Eraser Mini Columns and DNase I can remove trace DNA contaminants and obtain high purity and high yield RNA, which can be directly used in molecular biology experiments, such as RT-PCR, RT-qPCR, Northern blotting, NGS library construction, etc. The gDNA Eraser Mini Columns and DNase I can be used in molecular biology experiments.
| individual parts making up a compound | norm |
| Carrier RNA (100 ng / μl) | 100 μl X 3 pcs |
| DNase I (RNase free) | 200 μl |
| 10X DNase I Buffer | 1 ml |
| 50X DTT Solution | 350 μl |
| Buffer RLS | 22 ml |
| Buffer RWA | 35 ml |
| Buffer RWB*2 | 18 ml |
| RNase Free Water*3 | 5 ml |
| gDNA Eraser Mini Columns | 50 sets |
| RNA Micro Columns | 50 sets |
| Collection tubes | 50 pcs |
| RNase Free Tubes*4 | 50 pcs |
*1: Carrier RNA (100ng/μl), DNase I (RNase free), 10X DNase I Buffer and 50X DTT Solution are packaged in Package 2-1, and should be stored at -20℃, of which Carrier RNA (100 ng/μl) can also be stored at -80℃ for a long time. The Carrier RNA (100 ng/μl) can also be stored at -80℃ for a long period of time and avoid repeated freezing and thawing; the rest of the components are packaged in Package 2-2 and stored at room temperature (15 ~ 30℃).
*2: Before using Buffer RWB for the first time, please add 42 ml of anhydrous ethanol (the volume ratio of Buffer RWB to anhydrous ethanol is 3:7), mix it well, mark the bottle and store it at room temperature.
*3: RNase Free Water is recommended to be stored at -20 °C after opening.
*4: RNase Free Tubes are for elution of RNA only. Centrifuge tubes for pre-lysis should be provided.
Preservation Temperature:
Package 2-1 -20°C Storage
Package 2-2 Room Temperature (15-30°C) Storage
Transportation temperature:
Package 2-1 -20℃ Ice bag transportation or dry ice transportation
Package 2-2 Room Temperature (15-30°C) Transportation
