This product can be used to extract total RNA from biological samples such as less than 30 mg of animal tissues (liver, heart, kidney, spleen, intestines, etc.), less than 100 mg of plant tissues (stems, leaves, seeds, etc.), and less than 1.0E+07 cultured cells (HL60, 293T, etc.) etc. The kit adopts a unique cleavage system, which can be used to rapidly lyses the tissues or cells and inhibit the nucleases released from the cells without the need for toxic phenol-chloroform extraction. inhibiting nuclease released by the cells and maintaining RNA integrity. Total RNA is extracted by Universal RNA The Mini Column yields high purity, high yield RNA, and the purified Total RNA can be used directly in a variety of molecular biology experiments such as Northern hybridization, mRNA purification, in vitro translation, and RT-PCR. If necessary, DNase I (Code No. AG12001) can be used to treat Total RNA to remove trace amounts of DNA.
| individual parts making up a compound | norm |
| 50X DTT Solution*1 | 700 μl |
| DNase I (RNase free)*1 | 200 μl |
| 10X DNase I Buffer*1 | 1 ml |
| Buffer RLS | 15 ml × 2 |
| Buffer RWA | 30 ml |
| Buffer RWB*2 | 27 ml |
| RNase Free Water*3 | 13 ml |
| Universal RNA Mini Columns | 50 sets |
| Collection tubes | 50 pcs |
| RNase Free Tubes*4 | 50 pcs |
*1 These three components are packaged in Package 2-1 and should be stored at -20°C. The remaining components are packaged in Package 2-2 and should be stored at room temperature (15 - 25°C).
* 2 Buffer RWB Before first use, add 63 ml of 100% ethanol (3:7 Buffer RWB to anhydrous ethanol by volume), mix well, label the bottle and store at room temperature.
*3 Recommended storage at -20°C after opening.
*4 This component is for elution of RNA only; centrifuge tubes for pre-lysis should be provided.
Preservation Temperature:
Package 2-1 -20°C Storage;
Package 2-2 Store at room temperature (15 - 25°C).
Transportation temperature:
Package 2-1 Dry ice or -20°C ice packs for transportation;
Package 2-2 Room Temperature Transportation.
