SYBR Green Pro Taq HS Premixed qPCR Kit IV

Product Code: AG11746

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¥1,200

Product Description

This product is a special reagent for qPCR using SYBR Green I chimeric fluorescence method, it is a 2X Premix reagent, and the reaction solution is very simple to prepare. It is a 2X Premix reagent, and the reaction solution is easy to prepare. It is optimized for SYBR Green I concentration and PCR reaction system, and auxiliary proteins are added, which have obvious inhibiting effect on the production of primer dimer. Meanwhile, the PCR amplification efficiency is high, and it can carry out Real Time PCR reaction with high sensitivity, and get a good working curve in a wide quantitative region, so as to accurately quantify the target gene.

 

Product Advantages

①This product is a 2X premixed solution, premixed with SYBR Green I. The reaction solution is very simple to prepare, only need to add primers, templates and RNase free water to carry out qPCR reaction.
② The product is optimized for SYBR Green I concentration and PCR reaction system, and auxiliary proteins are added to make it have obvious inhibiting effect on the production of primer dimer.
③ The product has high detection sensitivity and can obtain good standard curve in a wide quantitative region, accurate quantitative detection of target genes with good reproducibility and high confidence.

Product Composition
individual parts making up a compound norm
2X SYBR Green Pro Taq HS Premix Ⅳ 1 ml x 5 pcs
Preservation and transportation
Storage temperature: -20℃ (keep away from light)
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
Example 1

This kit was used for fluorescence quantitative PCR detection of mouse GAPDH The amount of cDNA template added (equivalent to the amount of Total RNA) was 100 ng ~ 100 fg. cDNA was synthesized using our own cDNA template. Evo M-MLV Reverse transcription premix kit (contains gDNA removal reagents for qPCR) (Code. AG11728).
Quantitative instrument used: ABI QuantStudioTM 5 Real-Time PCR Systems. the results are shown below:

The results are shown above:
① Working curve R2= 0.9979, amplification efficiency 104%.
② Accurate quantification can be performed over a wide template range, with good linearity in the amplification curve over the range of 100 ng ~ 100 fg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.