SYBR Green <i>Pro Taq</i> HS 预混型 qPCR 试剂盒 II（含ROX）

SYBR Green Pro Taq HS Premixed qPCR Kit II (with ROX)

Product Code: AG11719

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¥1,200

Product Description

This product is a special reagent for qPCR using SYBR Green chimeric fluorescence method, and it is used in SYBR Green Premix. Pro Taq The HS qPCR Kit II (AG11702) is based on ROX's 2X Premix reagent, which is convenient to use. The SYBR Green concentration and PCR reaction system of this product have been optimized for high specificity and high PCR amplification efficiency, which can carry out Real Time PCR reaction with high sensitivity, and obtain a good standard curve within a wide quantification region, so as to accurately quantify and detect target genes.

Product Advantages

① This product is a 2X premixed solution, premixed with SYBR Green I. The reaction solution is very simple to prepare, just add primers, templates and RNase free water to carry out qPCR reaction.
② The product is optimized for SYBR Green I concentration and PCR reaction system, which is characterized by high amplification efficiency and strong amplification specificity.
③ This product is premixed with ROX Reference Dye, which effectively reduces the experimental error caused by the addition of ROX alone.

Product Composition
individual parts making up a compound norm
2X SYBR Green Pro Taq HS Premix II (Rox Plus) 1 ml x 5 pc
Preservation and transportation
Storage temperature: -20℃ (keep away from light, long-term storage at -20℃, the product can be stored at 4℃ for 1 month after melting.)
Transportation temperature: dry ice transportation
Example 1

This kit was used to perform a two-step fluorescence quantitative RT-PCR for the detection of Human GAPDH The amount of template cDNA added (equivalent to the amount of Total RNA) was 100 ng ~ 1 pg. cDNA was synthesized using our Evo M-MLV Reverse Transcription Kit (for qPCR) (Code. AG11706). The results are as follows:

The results are shown above:
①Working curve R2= 1.000, amplification efficiency 99.1%.
②Accurate quantification can be performed over a wide template range, with amplification curves showing good linearity over the range of 100 ng ~ 0.1 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

Example 2

Mouse was detected by two-step fluorescence quantitative RT-PCR using this kit. Hnf4a The amount of template cDNA added (equivalent to the amount of Total RNA) was 100 ng ~ 1 pg. cDNA was synthesized using our Evo M-MLV Reverse Transcription Kit (for qPCR) (Code. AG11706). The results are as follows:

The results are shown above:
①Working curve R2= 0.999, amplification efficiency 100%.
②Accurate quantification can be performed over a wide template range, and the amplification curve shows good linearity over the range of 100 ng ~ 1 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

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