SYBR Green <i>Pro Taq</i> HS 预混型 qPCR 试剂盒（含示踪染料）

SYBR Green Pro Taq HS Premixed qPCR Kit with Tracer Dye

Product Code: AG11733

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¥1,200

Product Description

This product is a special reagent for qPCR using SYBR Green chimeric fluorescence method. 2X Premix is added with blue dye, together with the yellow template diluent, which can realize the visualization of pipetting process: after mixing the yellow diluent with the template and adding it into the blue reaction solution, the solution will change from blue to green, and you can confirm whether to add the template or not according to the color change, which is beneficial for the addition of a large number of samples and reduces misoperation. It reduces the probability of misoperation.

In this product, the SYBR Green concentration and PCR reaction system were optimized, and the reaction performance of the superior Pro Taq HS system (mixed with Taq antibody) can effectively inhibit non-specific amplification, improve PCR amplification efficiency, can perform Real Time PCR reaction with high sensitivity, and can obtain a good standard curve in a wide quantitative region, so as to accurately quantify and detect target genes.

 

Product Advantages

① Blue dye is added to 2X Premix with yellow template diluent to visualize the pipetting process, improve the efficiency of sampling, and reduce the probability of mishandling.
② This product is a 2X premix, pre-mixed with SYBR Green I. The reaction solution is very simple to prepare, and only need to add primers, templates, and RNase free water to carry out qPCR reactions.
③ The product is optimized for SYBR Green I concentration and PCR reaction system, which is characterized by high amplification efficiency and strong amplification specificity.

Product Composition
individual parts making up a compound norm
2X SYBR Green Pro Taq HS Premix (Blue) 1 ml x 5 pc
40X Dilution Buffer (Yellow) 500 μl
Preservation and transportation
Storage temperature: -20℃ (keep away from light)
Transportation temperature: dry ice transportation
Example 1

This kit was used for the fluorescence quantitative RT-PCR detection of Mouse Hnf4a Gene (GC content 0%), and the amount of cDNA template added (equivalent to the amount of Total RNA) was 100 ng ~ 1 pg. cDNA was synthesized using the Company's Evo M-MLV Reverse Transcription Kit II (with gDNA removal reagent for qPCR) (Code.AG11711). Quantification instrument used: ABI QuantStudioTM 5 Real-Time PCR Systems. the results are shown below:

The results are shown above:
①Working curve R2= 0.9958, amplification efficiency 95.26%.
②Accurate quantification can be performed over a wide template range, with amplification curves showing good linearity over the range of 100 ng ~ 1 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

Example 2

The kit was used for the fluorescence quantitative RT-PCR method to detect Human β-Actin Gene (GC content 2%), and the amount of template cDNA added (equivalent to the amount of Total RNA) was 10 ng ~ 100 fg. cDNA was synthesized using our own cDNA synthesizers. Evo M-MLV Reverse Transcription Kit II (with gDNA removal reagent for qPCR) (Code.AG11711). The results were as follows: Quantification instrument used: CFX96 Real-Time PCR Detection System.

The results are shown above:
①Working curve R2= 0.9994, amplification efficiency 96.21%.
②Accurate quantification can be performed over a wide template range, with amplification curves showing good linearity over the range of 10 ng ~ 100 fg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

Example 3

This kit was used for the fluorescence quantitative RT-PCR detection of Human PARP1 Gene (GC content 4%), and the amount of cDNA template added (equivalent to the amount of Total RNA) was 10 ng ~ 1 pg. cDNA was synthesized using the Company's Evo M-MLV Reverse Transcription Kit II (with gDNA removal reagent for qPCR) (Code.AG11711). Quantification instrument used: CFX96 Real-Time PCR Detection System. results were as follows:

The results are shown above:
①Working curve R2= 0.9994, amplification efficiency 91.74%.
②Accurate quantification can be performed over a wide template range, with amplification curves showing good linearity over the range of 10 ng ~ 1 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

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