SYBR Green <i>Pro Taq</i> HS 预混型 qPCR 试剂盒 II（含示踪染料）

SYBR Green Pro Taq HS Premixed qPCR Kit II (with Tracer Dye)

Product Code: AG11736

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¥1,200

Product Description

This product is a special reagent for qPCR using SYBR Green chimeric fluorescence method, it is a 2X Premix reagent, easy to prepare the reaction solution. 2X Premix is added with a blue dye, together with the yellow template dilution buffer (40X Dilution Buffer (Yellow)), which can realize the visualization of the pipetting process: mix the yellow dilution buffer with the template and add it to the blue reaction solution, the solution will change from blue to green, which can be used to confirm whether the template is added according to the color change. After mixing the yellow dilution solution with the template, the solution will change from blue to green when added to the blue reaction solution, which can be used to confirm the addition of template according to the color change, which is beneficial to the addition of a large number of samples and reduces the probability of misuse.

In this product, the SYBR Green concentration and PCR reaction system have been optimized for high specificity and high PCR amplification efficiency, which can be used for Real Time PCR reaction with high sensitivity, and a good working curve can be obtained in a wide quantitative region, so as to accurately quantitatively detect the target genes.

Product Advantages

① Blue dye is added to 2X Premix with yellow template diluent to visualize the pipetting process, improve the efficiency of sampling, and reduce the probability of mishandling.
② This product is a 2X premixed solution, pre-mixed with SYBR. Green, the reaction solution is very simple to prepare, only need to add primers, template and RNase Free Water can be qPCR reaction.
③The product has a good effect on SYBR. The Green concentration and PCR reaction system were optimized to have high amplification efficiency and high amplification specificity.

 

Product Composition
individual parts making up a compound norm
2X SYBR Green Pro Taq HS Premix II (Blue) 1 ml x 5 pcs
40X Dilution Buffer (Yellow) 500 μl
Preservation and transportation
Storage temperature: -20℃ (keep away from light)
Transportation temperature: -20℃ Ice bag or dry ice transportation
Example 1

This kit was used for the fluorescence quantitative RT-PCR detection of Human βActin Gene (GC content 58.7%), and the amount of cDNA template added (equivalent to the amount of Total RNA) was 10 ng ~ 1 pg. cDNA was synthesized using the Company's Evo M-MLV Reverse transcription premix kit (with gDNA removal reagent for qPCR) (Code.AG11728). Quantification instrument used: ABI QuantStudioTM 5 Real-Time PCR Systems. results are shown below:

The results are shown above:
①Working curve R2= 0.9998, amplification efficiency 96.8%.
②Accurate quantification can be performed over a wide template range, with amplification curves showing good linearity over the range of 10 ng ~ 1 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

Example 2

The kit was used for the fluorescence quantitative RT-PCR method to detect Human TRF Gene (GC content 40.8%), and the amount of template cDNA added (equivalent to the amount of Total RNA) was 10 ng ~ 1 pg. cDNA was synthesized using our own cDNA synthesizer. Evo M-MLV Reverse transcription premix kit (with gDNA removal reagent for qPCR) (Code.AG11728). Quantification instrument used: CFX96 Real-Time PCR Detection System. results were as follows:

The results are shown above:
①Working curve R2= 0.9992, amplification efficiency 96.71 TP3T.
②Accurate quantification can be performed over a wide template range, and the amplification curves show good linearity over the range of 10 ng ~ 1 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

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