SYBR Green Taq HS 预混型 qPCR 试剂盒（含 UNG）

SYBR Green Taq HS Premixed qPCR Kit (with UNG)

Product Code: AG11756

- +

¥1,400

Product Description

This is a 2X premix type kit for qPCR using SYBR Green I chimeric fluorescence method. The reaction solution is very simple to prepare by adding primers, template and RNase free water. It is a 2X premix type reagent, and the reaction solution is easy to prepare, requiring only the addition of primers, template and RNase free water. Accurate Taq The HS DNA polymerase system enables highly sensitive Real Time PCR reactions for accurate quantification and detection of target genes.

The dUTP/UNG Anti-Contamination System is also introduced in this product, which replaces dTTP with dUTP in the PCR reaction, utilizing the fact that UNG enzyme can selectively hydrolyze dU-containing DNA strands without affecting dU-free DNA strands, and removes dU-containing contaminating templates that are introduced during the preparation of the PCR reaction system, thus preventing the generation of false-positive results of the PCR and improving the accuracy of experimental results. This can effectively prevent the generation of PCR false-positive results and improve the accuracy of experimental results.

 

Product Advantages

①This product is a 2X premixed solution, premixed with SYBR Green I. The reaction solution is very simple to prepare, only need to add primers, templates and RNase free water to carry out qPCR reaction.
② The product has been optimized for SYBR Green I concentration and PCR reaction system, featuring high amplification efficiency and strong amplification specificity.
The dUTP/UNG Contamination Prevention System has been introduced to remove contamination of dU-containing PCR products, thereby preventing false-positive PCR results and improving the accuracy of experimental results.

Product Composition
individual parts making up a compound norm
2X SYBR Green Taq HS Premix (UNG Plus)* 1 ml x 5 pcs

*: White or yellowish precipitate may occur when the solution is stored at -20°C. Dissolve on ice or by hand before use and mix upside down until all precipitate disappears. Do not vortex and oscillate.

Preservation and transportation
Storage temperature: -20℃ storage (keep away from light)
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
Example 1

This kit was used for the fluorescence quantitative PCR detection of Mouse GAPDH The amount of cDNA template added (equivalent to the amount of Total RNA) was 100 ng ~ 1 pg. cDNA was synthesized using our own cDNA template. Evo M-MLV Reverse Transcription Premix Kit (with gDNA removal reagent for qPCR) Ver.2 (Code No. AG11728). The quantification instrument used was the ABI QuantStudioTM 5 Real-Time PCR Systems. the results are shown below:

The results are shown above:
① Working curve R2= 1.000, amplification efficiency 101.21 TP3T.
② Accurate quantification can be performed over a wide template range, with good linearity in the amplification curve over the range of 100 ng ~ 1 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type and good amplification specificity.

Example 2

This kit was used for the fluorescence quantitative PCR detection of Mouse malat1 The cDNA template was added in amounts of 100 ng ~ 1 pg of total RNA. cDNA was synthesized using the Evo M-MLV Reverse Transcription Premix Kit (with gDNA removal reagent for qPCR) Ver.2 (Code No. AG11728). The quantification instrument used was the ABI QuantStudioTM 5 Real-Time PCR Systems. the results are shown below:

The results are shown above:
① Working curve R2= 0.999, amplification efficiency 101.21 TP3T.
② Accurate quantification can be performed over a wide template range, with good linearity in the amplification curve over the range of 100 ng ~ 1 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type and good amplification specificity.

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