{"id":2555,"date":"2023-03-29T11:32:36","date_gmt":"2023-03-29T03:32:36","guid":{"rendered":"https:\/\/en.agbio.com.cn\/?page_id=2555"},"modified":"2023-05-11T12:17:20","modified_gmt":"2023-05-11T04:17:20","slug":"oligo-synthesis-faq","status":"publish","type":"page","link":"https:\/\/en.agbio.com.cn\/en\/oligo-synthesis-faq\/","title":{"rendered":"Synthesis FAQ"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"2555\" class=\"elementor elementor-2555\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-1f2a812d elementor-section-full_width elementor-section-height-min-height elementor-section-height-default elementor-section-items-middle\" data-id=\"1f2a812d\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t\t<div class=\"elementor-background-overlay\"><\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-5a10d63f\" data-id=\"5a10d63f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-1d96952e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"1d96952e\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-1522077a\" data-id=\"1522077a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5fa27f95 elementor-widget elementor-widget-heading\" data-id=\"5fa27f95\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Oligo Synthesis FAQ<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-25990a44 elementor-widget__width-auto e-transform elementor-widget elementor-widget-button\" data-id=\"25990a44\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:10,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/en.agbio.com.cn\/en\/wp-content\/pluguns\/chat.html\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Online Customer Service<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-c83a0c3 elementor-section-content-bottom animated-slow elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-invisible\" data-id=\"c83a0c3\" data-element_type=\"section\" data-settings=\"{&quot;animation&quot;:&quot;fadeIn&quot;,&quot;animation_delay&quot;:400}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-11a4596c\" data-id=\"11a4596c\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-1c67e90f elementor-widget elementor-widget-heading\" data-id=\"1c67e90f\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Oligo Synthesis FAQ Catalog:<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5ec0df40 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"5ec0df40\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-3ca65161 elementor-section-full_width elementor-section-height-default elementor-section-height-default\" data-id=\"3ca65161\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-101eadcd\" data-id=\"101eadcd\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-1643062c elementor-widget elementor-widget-text-editor\" data-id=\"1643062c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li><a href=\"#faq-question-1\">What does OD value mean?<\/a><\/li><li><a href=\"#faq-question-2\">How is synthesized DNA \/ RNA quantified?<\/a><\/li><li><a href=\"#faq-question-3\">How is the OD value of synthesized DNA \/ RNA determined and calculated?<\/a><\/li><li><a href=\"#faq-question-4\">How are synthesized DNA dry powders diluted and preserved?<\/a><\/li><li><a href=\"#faq-question-5\">How are synthesized RNA dry powders diluted and preserved?<\/a><\/li><li><a href=\"#faq-question-6\">How do I dissolve Oligo DNA\/RNA smaller than 15 mer?<\/a><\/li><li><a href=\"#faq-question-7\">How are synthesized Oligo products electrophoresed?<\/a><\/li><li><a href=\"#faq-question-8\">How are synthesized fluorescently labeled probes stored?<\/a><\/li><li><a href=\"#faq-question-9\">Will a synthesized DNA solution degrade if left at room temperature for a few days?<\/a><\/li><li><a href=\"#faq-question-10\">Oligo solution has OD260 \/ OD280 &lt; 1.8, does this mean the solution is impure?<\/a><\/li><li><a href=\"#faq-question-11\">What are the purification levels of Oligo products? What is the difference?<\/a><\/li><li><a href=\"#faq-question-12\">How do you calculate the molarity of a synthesized Oligo DNA product?<\/a><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-5c95861a\" data-id=\"5c95861a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-40045de0 elementor-widget elementor-widget-text-editor\" data-id=\"40045de0\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li><a href=\"#faq-question-13\">The synthesized Oligo was dissolved and found to have a small amount of precipitate, will this affect subsequent experiments?<\/a><\/li><li><a href=\"#faq-question-14\">When Oligo DNA of the same length is subjected to PAGE electrophoresis, why are the positions of the bands inconsistent?<\/a><\/li><li><a href=\"#faq-question-15\">Is there a phosphate group at the 5\u2032 and 3\u2032 ends of synthetic Oligo DNA?<\/a><\/li><li><a href=\"#faq-question-16\">When performing antisense nucleic acid experiments, do you need to modify all of the DNA strands with S substitutions, and are there other ways to increase the stability of nucleic acids besides S substitutions?<\/a><\/li><li><a href=\"#faq-question-17\">What principles should be followed in the design of dual-labeled fluorescent probes?<\/a><\/li><li><a href=\"#faq-question-18\">How should quenching groups and fluorescent groups be paired when modifying fluorescent probes?<\/a><\/li><li><a href=\"#faq-question-19\">How should fluorescent group modifications be selected for multiprobe assays?<\/a><\/li><li><a href=\"#faq-question-20\">How to choose the quenching group of TAMRA and BHQ?<\/a><\/li><li><a href=\"#faq-question-21\">What is the difference between FITC, 6-FAM, and 5-FAM markers?<\/a><\/li><li><a href=\"#faq-question-22\">The PCR product was cloned and sequenced and found to have an error in a base at the primer.<\/a><\/li><li><a href=\"#faq-question-23\">Why are flat PCR products harder to clone?<\/a><\/li><li><a href=\"#faq-question-24\">What is the length of DNA \/ RNA that can be synthesized by Aceri?<\/a><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-3f6fc420 elementor-section-full_width elementor-section-height-min-height animated-slow elementor-section-height-default elementor-section-items-middle elementor-invisible\" data-id=\"3f6fc420\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;,&quot;animation&quot;:&quot;fadeIn&quot;,&quot;animation_delay&quot;:300}\">\n\t\t\t\t\t\t\t<div class=\"elementor-background-overlay\"><\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-2bda3f1f\" data-id=\"2bda3f1f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-f0cb3d5 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"f0cb3d5\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-49db22e6\" data-id=\"49db22e6\" data-element_type=\"column\" id=\"faq-question-1\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-26f31583 elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"26f31583\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div 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class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tOD is an abbreviation for optical density, which indicates the density of light absorbed by the detected object.\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-52184b9d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"52184b9d\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-5b6f73fd\" data-id=\"5b6f73fd\" data-element_type=\"column\" id=\"faq-question-2\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-79f95293 elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"79f95293\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 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35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tHow is synthesized DNA \/ RNA quantified?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tBecause nucleic acids absorb strongly near 260 nm, they can be quantified by UV spectrophotometry based on this property and expressed as an OD value.1 OD means that if a solution of DNA\/RNA is placed in a cuvette with an optical path length of 1 cm, and if its absorbance at 260 nm is 1, then the amount of DNA\/RNA dissolved in 1 ml of the solution is said to be 1 OD. The mass of synthesized DNA\/RNA for one OD is approximately 33 \u03bcg (the unit of total amount, not concentration). The mass of synthesized DNA\/RNA for 1 OD is approximately 33 \u03bcg (OD is a unit of total amount, not concentration). \t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-5c9812d3 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"5c9812d3\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-481491d9\" data-id=\"481491d9\" data-element_type=\"column\" id=\"faq-question-3\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-76aed438 elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"76aed438\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tHow is the OD value of synthesized DNA \/ RNA determined and calculated?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tThe DNA\/RNA solution is diluted appropriately so that the absorbance measurement is within the precise range allowed by the instrument, and then the total OD value of the solution is calculated based on the total volume of the original solution and the number of times it was diluted. For example, if a 300 \u03bcl DNA\/RNA solution is diluted 5-fold and the measured value is 1.0, the total OD value of the solution is: 0.3 ml \u00d7 5 \u00d7 1.0 OD \/ ml = 1.5 OD (if the measured value is greater than 1.5, it is recommended to re-dilute the solution before measurement in order to ensure the accuracy of the measurement).\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-d275a91 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"d275a91\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-1590a66f\" data-id=\"1590a66f\" data-element_type=\"column\" id=\"faq-question-4\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-fb612ae elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"fb612ae\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tHow are synthesized DNA dry powders diluted and preserved?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tFor long term storage, it is recommended to store the dry powder directly or dissolve it in sterile TE buffer (10 mM Tris pH 8.0, 0.1 M EDTA) to a higher concentration (e.g., 100 \u00b5M) and store at -20\u00b0C or -80\u00b0C, avoiding repeated freezing and thawing (do not store in a refrigerator with an automatic defrost function). It can also be diluted in sterile, enzyme-free water or to a working concentration (10 \u00b5M), but the effective shelf-life will be shortened.\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-ec5a079 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"ec5a079\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-448cae77\" data-id=\"448cae77\" data-element_type=\"column\" id=\"faq-question-5\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3597f282 elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"3597f282\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tHow are synthesized RNA dry powders diluted and preserved?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tThe dry powder is recommended to be stored at -80\u00b0C, if not, please store at -20\u00b0C. Usually it can be stored for 2 years. RNA in solution state is best stored at -80\u00b0C, if not, store at -20\u00b0C.\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-6d95e238 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"6d95e238\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-12312cca\" data-id=\"12312cca\" data-element_type=\"column\" id=\"faq-question-6\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-27195cf5 elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"27195cf5\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tHow do I dissolve Oligo DNA\/RNA smaller than 15 mer?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tOligo DNA \/ RNA length \u226415 mer or sequence-specific cases will have reduced solubility (Milli-Q water may be insoluble) and TE (pH 7.5 - 8.0) is recommended for solubilization.\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-2a893a0e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2a893a0e\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-723c0e79\" data-id=\"723c0e79\" data-element_type=\"column\" id=\"faq-question-7\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3a9f9829 elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"3a9f9829\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tHow are synthesized Oligos products electrophoresed?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tSingle-stranded Oligo tends to form complex three-dimensional structures and is therefore not suitable for agarose electrophoresis (multiple bands may appear) and should not be stained with EB, GoldView or CelRed. Electrophoresis should be performed using 15% Denaturing PAGE Gel, and visualized by imaging on a silica gel fluorescent plate with a UV lamp (254 nm).\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-9d1aa91 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"9d1aa91\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-fbd6d40\" data-id=\"fbd6d40\" data-element_type=\"column\" id=\"faq-question-8\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-54b5e47f elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"54b5e47f\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tHow are synthesized fluorescently labeled probes stored?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tFluorescent probes should be stored under strict light protection; dry powder can be stored at -20\u2103 for 2 years without degradation, but care should be taken to avoid repeated freezing and thawing.\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-1c9695af elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"1c9695af\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-6a564f9c\" data-id=\"6a564f9c\" data-element_type=\"column\" id=\"faq-question-9\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-370e34a5 elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"370e34a5\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tWill a synthesized DNA solution degrade if left at room temperature for a few days?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tIn general, a 100 \u00b5M concentration of Oligo DNA solution at room temperature (25\u00b0C - 30\u00b0C) for 3-4 days has little effect, but it is best not to leave it for more than a week. The exact amount of time depends on the degree of sterilization of the synthesis vessels and reagents.\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-dcde934 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"dcde934\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-65367332\" data-id=\"65367332\" data-element_type=\"column\" id=\"faq-question-10\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4efd7ab4 elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"4efd7ab4\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tOligo solution has OD260 \/ OD280 &lt; 1.8, does this mean the solution is impure?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tNucleic acids have strong absorption near 260 nm, and proteins have strong absorption near 280 nm. When the proportions of A, G, C, and T in the sequences are roughly the same, the OD260 \/ OD280 ratio can be used to evaluate whether there is any protein residue in the nucleic acids (the ratio is in the range of 1.8 to 2.2), such as nucleic acids extracted from organisms. However, the synthesized Oligo sequence is very short, in which A, G, C, and T bases account for different proportions, and since the molar extinction coefficients of the bases are different, the OD260\/OD280 ratios of Oligo sequences composed of different bases are also different. For example, when the sequence has a high content of C and T bases, the ratio is much lower than 1.8. In addition, the order of the bases in the sequence also affects the ratio. Therefore, it is not possible to evaluate the purity of synthesized Oligo based on the OD260 \/ OD280 ratio.\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-51ab56bb e-transform elementor-widget elementor-widget-html\" data-id=\"51ab56bb\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"html.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<table>\n    <tr style=\"text-align:center;\">\n        <td style=\"width:50%;background-color:#005BAC;color:#ffffff;\"><b>base percentage<\/b><\/td>\n        <td style=\"width:50%;background-color:#005BAC;color:#ffffff;\"><b>OD260 \/ OD280<\/b><\/td>\n    <\/tr>\n        <tr style=\"text-align:center;\">\n        <td style=\"width:50%;\">100% A Base<\/td>\n        <td style=\"width:50%;\">2.5<\/td>\n    <\/tr>\n        <tr style=\"text-align:center;\">\n        <td style=\"width:50%;\">100% G Base<\/td>\n        <td style=\"width:50%;\">1.85<\/td>\n    <\/tr>\n        <tr style=\"text-align:center;\">\n        <td style=\"width:50%;\">100% C Base<\/td>\n        <td style=\"width:50%;\">1.15<\/td>\n    <\/tr>\n        <tr style=\"text-align:center;\">\n        <td style=\"width:50%;\">100% T Base<\/td>\n        <td style=\"width:50%;\">1.14<\/td>\n    <\/tr>\n        <tr style=\"text-align:center;\">\n        <td style=\"width:50%;\">A, G, C, T (25% each)<\/td>\n        <td style=\"width:50%;\">1.66<\/td>\n    <\/tr>\n<\/table>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-799d7256 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"799d7256\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-16c11004\" data-id=\"16c11004\" data-element_type=\"column\" id=\"faq-question-11\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-73e824a1 elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"73e824a1\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tWhat are the purification levels of Oligo products? What is the difference?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tI. Desalting grade: Simple desalting to remove salts and ions generated during synthesis, suitable for shorter sequences with less demanding downstream experiments.<br \/>\nSecond, OPC purification grade: the small column of C18 resin is used for purification, which can remove the short-stranded DNA that fails to be synthesized.<br \/>\nPAGE level: According to the charge and molecular size, the target sequence can be distinguished from the failed longer sequences, and within a certain range, it can effectively remove the N-1 impurity chain.<br \/>\nHPLC grade: It can separate N-1 impurities more effectively through the combined purification of ion exchange column and reverse C18 column.\n\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-75647571 e-transform elementor-widget elementor-widget-html\" data-id=\"75647571\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"html.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<table>\n    <tr style=\"text-align:center;\">\n        <td style=\"width:25%;background-color:#005BAC;color:#ffffff;\"><b>Purification method<\/b><\/td>\n        <td style=\"width:20%;background-color:#005BAC;color:#ffffff;\"><b>appliance<\/b><\/td>\n                <td style=\"width:25%;background-color:#005BAC;color:#ffffff;\"><b>Range of purified bases\n(bases)\n<\/b><\/td>\n                        <td style=\"width:25%;background-color:#005BAC;color:#ffffff;\"><b>fineness<\/b><\/td>\n    <\/tr>\n        <tr style=\"text-align:center;\">\n        <td>desalination<\/td>\n        <td>Sequencing, conventional PCR, hybridization DNA microarrays, SNP analysis, etc.<\/td>\n        <td>10 - 60<\/td>\n        <td>Related to sequence length<\/td>\n    <\/tr>\n        <tr style=\"text-align:center;\">\n        <td>OPC level<\/td>\n        <td>AFLP, OLA, diagnostic PCR, antisense nucleic acids, traditional modifications, etc.<\/td>\n        <td>2 - 60<\/td>\n        <td>80% Above<\/td>\n    <\/tr>\n        <tr style=\"text-align:center;\">\n        <td>PAGE Level<\/td>\n        <td>Primers for molecular diagnostics, quantitative PCR, cloning, subcloning PCR, gene synthesis, gel migration evaluation, etc.<\/td>\n        <td>10 or more<\/td>\n        <td>90% - 95%<\/td>\n    <\/tr>\n        <tr style=\"text-align:center;\">\n        <td>HPLC grade<\/td>\n        <td>In situ hybridization, miRNA, siRNA, quantitative PCR, capillary sequencing, primers for molecular diagnostics, etc.<\/td>\n        <td>2 - 60<\/td>\n        <td>95% Above<\/td>\n    <\/tr>\n        <tr style=\"text-align:center;\">\n        <td>HPLC + PAGE grade<\/td>\n        <td>X-Ray Crystallographic studies, NMR, 3D structural studies, difficult cloning and gene synthesis, etc.<\/td>\n        <td>10 or more<\/td>\n        <td>Highest purity<\/td>\n    <\/tr>\n<\/table>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-12fc5a62 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"12fc5a62\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-59278961\" data-id=\"59278961\" data-element_type=\"column\" id=\"faq-question-12\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5b5ddd4f elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"5b5ddd4f\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tHow do you calculate the molarity of a synthesized Oligo DNA product?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tSince each base has a different light-absorbing capacity (molar extinction coefficient \u03b5260), a more accurate formula for calculating the Oligo DNA nmol number is as follows:<br \/>\nnmol number = total solution OD * 10<sup>6<\/sup> \/ \u03b5260<br \/>    \u03b5260 = (15.2 * number of A bases) + (11.5 * number of G bases) + (7.4 * number of C bases) + (8.3 * number of T bases)\n\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-3c0d7654 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3c0d7654\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-1b0948a3\" data-id=\"1b0948a3\" data-element_type=\"column\" id=\"faq-question-13\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6d7dde16 elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"6d7dde16\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tThe synthesized Oligo was dissolved and found to have a small amount of precipitate, will this affect subsequent experiments?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tOccasionally, trace amounts of C18 resin may enter the product during OPC purification. The resin does not affect any of the reaction results and can be centrifuged slightly and the supernatant used.\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-55c5d2f1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"55c5d2f1\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-15458a59\" data-id=\"15458a59\" data-element_type=\"column\" id=\"faq-question-14\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5cfa2785 elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"5cfa2785\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tWhen Oligo DNA of the same length is subjected to PAGE electrophoresis, why are the positions of the bands inconsistent?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tDifferent base compositions result in inconsistent electrophoretic speeds for Oligo DNA; different DNA stereo structures also have different electrophoretic speeds. (Usually occurs in shorter sequences, with less variation among long-stranded Oligo DNA).\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-4af17416 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4af17416\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-5ff00bad\" data-id=\"5ff00bad\" data-element_type=\"column\" id=\"faq-question-15\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-21bd7d4f elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"21bd7d4f\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 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35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tIs there a phosphate group at the 5' and 3' ends of synthetic Oligo DNA?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tIf not required, the synthesized Oligo DNA is normally -OH without a phosphate group at the end. If an additional phosphate group is required, it can be specified when placing the order, in which case the cost of phosphorylation (PO4 modification) will be charged.\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-3ab7a81b elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3ab7a81b\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-207ccb1d\" data-id=\"207ccb1d\" data-element_type=\"column\" id=\"faq-question-16\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2ac9fa6e elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"2ac9fa6e\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tWhen performing antisense nucleic acid experiments, is it necessary to modify all of the DNA strand with the S-substitution, and is there any other way to increase the stability of nucleic acids besides the S-substitution?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tS-substitution modification increases the stability of the nucleic acid in the cell and prevents degradation by nuclease. However, complete modification of the entire strand is not recommended (except for injection into live animals), as this will reduce the Tm value and the binding efficiency of the antisense DNA to the target sequence. Phosphorothioated Bonds can be inserted at both ends of the DNA fragments to increase both the stability of the DNA and the ability of the antisense DNA to bind to the target sequence.<br \/><br \/>\nIn addition to S-generation modifications, 2' - OMe - RNA can also be used to increase stability by forming a chimeric antisense nucleic acid with S-generation DNA, which both enhances the stability of the antisense nucleic acid and increases the affinity for the target sequence (the affinity of 2' - OMe - RNA for RNA is much greater than that of DNA for RNA). In addition, 5 - Me - dC can also be added to S-generation antisense nucleic acids due to its ability to enhance the stability of the DNA double helix.\n\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-1b52a187 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"1b52a187\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-294068bb\" data-id=\"294068bb\" data-element_type=\"column\" id=\"faq-question-17\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-59385291 elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"59385291\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tWhat principles should be followed in the design of dual-labeled fluorescent probes?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\t\u25cf The probe should be located between the two primers and can be either a forward or reverse probe;<br \/>\n\u25cf The content of bases G and C in the probe should preferably be controlled between 40% and 60%; avoid strings of the same base, especially base G;<br \/>\n\u25cf Base G should not appear at the 5' end; the Tm value of the probe should be 8-10\u2103 higher than that of the primer, preferably between 68 and 70\u2103;<br \/>\n\u25cf When the length of the probe exceeds 30 bases, it is recommended to put the quenching group in the middle to prevent the fluorescence background from being too high, and then the 3' end of the probe should be blocked with a phosphoryl group to prevent the probe from extending during the PCR reaction.\n\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-3f3cdc09 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3f3cdc09\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-285206b6\" data-id=\"285206b6\" data-element_type=\"column\" id=\"faq-question-18\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-28501bf3 elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"28501bf3\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tHow should quenching groups and fluorescent groups be paired when modifying fluorescent probes?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tThe role of the quenching group is to quench the fluorescence of the reporter group, so the fluorescence spectrum of the quenching group should be similar to the absorption spectrum of the reporter group. Reference can be made to the following figure:\n\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-29610e93 e-transform elementor-widget elementor-widget-image\" data-id=\"29610e93\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/en.agbio.com.cn\/wp-content\/uploads\/2023\/03\/\u56fe\u72471-600x373-1.png\" title=\"Image 1-600\u00d7373\" alt=\"\u56fe\u72471-600x373\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-46577ca5 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"46577ca5\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-5df09a63\" data-id=\"5df09a63\" data-element_type=\"column\" id=\"faq-question-19\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3b01c71a elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"3b01c71a\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tHow should fluorescent group modifications be selected for multiprobe assays?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tThe traditional fluorescence quantitative PCR instrument is usually four fluorescence detection channels, each channel detects different fluorescence signals, can refer to the following table to match:\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7dc83eb2 e-transform elementor-widget elementor-widget-html\" data-id=\"7dc83eb2\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"html.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<table>\n    <tr style=\"text-align:center;\">\n        <td style=\"width:25%;background-color:#005BAC;color:#ffffff;\"><b> <\/b><\/td>\n        <td style=\"width:25%;background-color:#005BAC;color:#ffffff;\"><b>excitation wavelength<\/b><\/td>\n        <td style=\"width:25%;background-color:#005BAC;color:#ffffff;\"><b>Detection wavelength<\/b><\/td>\n        <td style=\"width:25%;background-color:#005BAC;color:#ffffff;\"><b>fluorescent marker<\/b><\/td>\n    <\/tr>\n        <tr style=\"text-align:center;\">\n        <td>Channel 1<\/td>\n        <td>450 - 490 nm<\/td>\n        <td>515 - 530 nm<\/td>\n        <td>FAM, SYBR Green<\/td>\n    <\/tr>\n        <tr style=\"text-align:center;\">\n        <td>second channel<\/td>\n        <td>500 - 535 nm<\/td>\n        <td>560 - 580 nm<\/td>\n        <td>vic, hex, toe, tamra, tet, cy3<\/td>\n    <\/tr>\n        <tr style=\"text-align:center;\">\n        <td>third channel<\/td>\n        <td>555 - 585 nm<\/td>\n        <td>610 - 650 nm<\/td>\n        <td>ROX, Texas-Red<\/td>\n    <\/tr>\n        <tr style=\"text-align:center;\">\n        <td>Corridor 4<\/td>\n        <td>620 - 650 nm<\/td>\n        <td>675 - 730 nm<\/td>\n        <td>Cy5<\/td>\n    <\/tr>\n<\/table>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-4c0135d9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4c0135d9\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-3f97b542\" data-id=\"3f97b542\" data-element_type=\"column\" id=\"faq-question-20\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-22eab047 elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"22eab047\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tHow to choose the quenching group of TAMRA and BHQ?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tTAMRA is a fluorescent dye and emits its own fluorescence at higher wavelengths, so the probe fluorescence background is relatively high. The BHQ series are non-fluorescent dyes that only quench the reporter group and do not emit fluorescence themselves, resulting in a low probe fluorescence background, a higher signal-to-noise ratio, and higher detection sensitivity.<br \/><br \/>\nTAMRA has a narrow absorption spectrum with fewer types of quenching groups, whereas the BHQ series of dyes have a wider absorption spectrum with more types of quenching groups. Therefore, the BHQ series of dyes are commonly used for quenching moieties in multiplex PCR assays.\n\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-7cabc575 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7cabc575\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-9672ca4\" data-id=\"9672ca4\" data-element_type=\"column\" id=\"faq-question-21\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2ea0eabd elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"2ea0eabd\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tWhat is the difference between FITC, 6-FAM, and 5-FAM markers?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tAll three are fluorescein-labeled (Fluorescein), and the chromophore is fluorescein; however, structurally, 5 - FAM and 6 - FAM are isomers of each other; FITC is linked to Oligo by a thiourea bond), whereas the former is an amide bond, and there is no difference in usual use.\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3baf913d e-transform elementor-widget elementor-widget-image\" data-id=\"3baf913d\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/en.agbio.com.cn\/wp-content\/uploads\/2023\/03\/\u56fe\u72472-600x377-1.jpg\" title=\"Image 2-600\u00d7377\" alt=\"\u56fe\u72472-600x377\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-463c37a7 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"463c37a7\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-76b84bd4\" data-id=\"76b84bd4\" data-element_type=\"column\" id=\"faq-question-22\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-668b5dc9 elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"668b5dc9\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tThe PCR product was cloned and sequenced and found to have an error in a base at the primer.\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tBase errors at primers can be troubleshooted in several ways:<br \/><br \/>\n\u25cf Artificially entering the sequence incorrectly (synthetic report card can be checked);<br \/>\u25cf Whether the purification level is suitable for downstream experiments;<br \/>\u25cf Reselect clones for sequencing (primer purity is unlikely to be 100%; it is possible that clones with PCR products amplified by impurity primers were selected).<br \/><br \/>\nIf there is still a problem through the above troubleshooting, Acres will re-synthesize the primers free of charge.\n\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-19151904 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"19151904\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-626bf889\" data-id=\"626bf889\" data-element_type=\"column\" id=\"faq-question-23\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5cb94e4b elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"5cb94e4b\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 0-30.637788-13.676487-30.637788-30.635741L469.820853 571.606563l71.483054 0c16.95209 0 30.637788-13.684674 30.637788-30.631648l0-71.495334 153.184845 0c16.959254 0 30.637788 13.690814 30.637788 30.643927L755.764328 724.793454zM499.191788 482.500129c-1.738597-1.638313-5.41022-3.683902-10.82658-6.434549-5.41022-2.558265-10.519588-5.41636-15.008832-8.275477 12.764722-15.014972 19.294438-35.542494 19.294438-61.48126 0-17.055444-3.15997-31.866778-9.592472-44.425815-6.331195-12.656251-15.732309-22.363334-28.189016-29.207206-12.45773-6.945179-27.371394-10.417257-44.528146-10.417257-12.764722 0-24.102954 1.943258-34.213219 5.723352-10.007935 3.876284-18.481933 9.29162-25.52842 16.33913-6.945179 7.14984-12.253068 15.72617-15.929808 25.837458-3.676739 10.212596-5.41636 21.236674-5.41636 33.291221 0 11.946077 1.739621 22.978341 5.41636 33.190937 3.575432 10.212596 8.984628 18.896372 16.134469 26.244734 7.142677 7.252171 16.134469 13.07683 26.654057 16.84976 10.519588 3.984754 22.363334 5.921873 35.742039 5.921873 16.236799 0 30.127158-2.039449 41.46539-6.325056 5.41022 3.773953 10.519588 7.143701 15.315824 10.207479 4.598738 2.660596 8.582469 4.903683 12.156878 6.54302 3.473101 1.426489 6.332219 2.14178 8.883321 2.14178 2.864234 0 5.51869-1.125637 7.661494-2.960425 2.14178-2.251274 3.261277-4.290723 3.261277-6.228865C501.942435 486.170728 500.925269 484.027925 499.191788 482.500129zM458.341405 430.310489c-2.346441 7.66047-6.024204 14.190187-11.133571 19.606547-17.567098-12.150738-30.024827-18.174941-37.378306-18.174941-2.552126 0-4.796236 0.913812-6.734378 2.654456-2.046612 1.943258-2.967588 4.079922-2.967588 6.228865 0 2.961448 0.920976 5.006014 3.165086 6.432503 2.046612 1.63729 6.031367 3.677762 11.850909 6.331195 5.716188 2.76395 10.513448 5.51869 14.292518 8.173147-6.331195 2.552126-12.56006 3.677762-18.686594 3.677762-14.606673 0-26.961048-5.314029-36.45733-15.929808-9.598612-10.723226-14.394848-26.047236-14.394848-45.859467 0-9.496282 1.220804-18.276249 3.876284-26.14138 2.448772-7.867178 6.024204-14.19735 10.519588-19.307741 4.393054-4.898567 9.700943-8.773827 15.929808-11.33107 6.024204-2.552126 12.764722-3.984754 20.1182-3.984754 10.212596 0 19.095917 2.347464 27.063379 7.14984 7.866155 4.802376 13.889335 11.645225 18.283412 20.936845 4.079922 9.292644 6.331195 20.221554 6.331195 32.883945C462.018144 413.869029 460.790176 422.647973 458.341405 430.310489zM648.700917 574.408375c-2.040473-5.102205-3.77907-9.087982-5.109368-12.150738-1.220804-2.961448-2.858094-5.819542-4.797259-8.582469-1.937119-2.551102-4.393054-4.796236-7.455809-6.53688-3.166109-1.63115-7.04137-2.550079-11.639085-2.550079-4.700045 0-8.480138 0.918929-11.645225 2.550079-3.267417 1.740644-5.614881 3.985778-7.552 6.747681-1.841951 2.654456-3.787256 6.018064-5.417383 10.212596-1.739621 4.079922-3.166109 7.65433-4.290723 10.512425L548.51617 677.150364c-1.732457 4.183276-3.062755 7.553023-3.677762 9.804297-0.818645 2.243087-1.125637 4.489244-1.125637 6.534833 0 3.77293 1.63729 7.354502 4.802376 10.212596s7.04751 4.285606 11.134595 4.285606c4.999874 0 8.472975-1.221828 10.723226-4.183276 2.24411-2.864234 4.797259-8.275477 7.961322-16.242939l7.866155-20.419052 67.096141 0 7.860015 20.1182c0.919952 2.552126 2.24411 5.41022 3.8814 8.576329 1.432629 3.166109 2.865257 5.613858 4.087085 7.252171 1.330298 1.527796 2.75474 2.653433 4.596691 3.676739 1.938142 0.920976 4.183276 1.221828 6.945179 1.221828 4.386914 0 8.268314-1.426489 11.434423-4.490268 3.166109-2.965541 4.802376-6.331195 4.802376-10.211573 0-3.473101-1.738597-9.094122-5.109368-16.850783L648.700917 574.408375zM594.983481 646.710075l24.409946-71.482031 24.812105 71.482031L594.983481 646.710075z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tWhy are flat PCR products harder to clone?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tTypical PCR primers do not have a phosphate group at the 5\u2032 end, and therefore the amplified PCR product does not have a phosphate group at the 5\u2032 end. When cloned into dephosphorylated end-smoothing vectors, they cannot be cloned in, and when cloned into non-dephosphorylated end-smoothing vectors, the background is extremely high. This can be achieved by phosphorylating (PO4-modifying) the 5\u2032 end of the PCR product.\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-58745752 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"58745752\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-3062f016\" data-id=\"3062f016\" data-element_type=\"column\" id=\"faq-question-24\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-63e7901b elementor-position-inline-start e-transform elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"63e7901b\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_hover_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" class=\"icon\" viewbox=\"0 0 1024 1024\" width=\"200\" height=\"200\"><defs><style type=\"text\/css\"><\/style><\/defs><path d=\"M510.671237 62.821791c-247.146866 0-447.515337 200.361307-447.515337 447.509197 0 247.153006 200.36847 447.51329 447.515337 447.51329 247.14789 0 447.508173-200.360284 447.508173-447.51329C958.17941 263.183098 757.819127 62.821791 510.671237 62.821791zM265.56996 520.549723 265.56996 295.868521c0-16.946974 13.685697-30.632671 30.631648-30.632671l224.682225 0c16.946974 0 30.632671 13.685697 30.632671 30.632671l0 224.681202c0 16.945951-13.685697 30.630624-30.632671 30.630624L296.202631 551.180348C279.255657 551.180348 265.56996 537.495674 265.56996 520.549723zM755.765351 724.793454c0 16.959254-13.678534 30.635741-30.637788 30.635741L500.458641 755.429195c-16.95209 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Aceri?\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tThe length of synthesized DNA ranges from 1 to 200 bases; the length of synthesized RNA ranges from 1 to 120 bases. All products use TOF-MS for molecular weight determination to ensure the accuracy of synthesis (when synthesized DNA sequences are long, it is difficult to ensure that each base is correct due to the limitations of synthesis and purification methods).\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Oligo \u5408\u6210\u5e38\u89c1\u95ee\u9898 \u5728\u7ebf\u5ba2\u670d Oligo \u5408\u6210\u5e38\u89c1\u95ee\u9898\u76ee\u5f55\uff1a OD \u503c\u662f\u4ec0\u4e48\u610f\u601d\uff1f \u5408\u6210\u7684 DNA \/  [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"slim_seo":{"title":"\u5408\u6210 FAQ - Accurate Biology","description":"Oligo \u5408\u6210\u5e38\u89c1\u95ee\u9898 \u5728\u7ebf\u5ba2\u670d Oligo \u5408\u6210\u5e38\u89c1\u95ee\u9898\u76ee\u5f55\uff1a OD \u503c\u662f\u4ec0\u4e48\u610f\u601d\uff1f \u5408\u6210\u7684 DNA \/ RNA \u662f\u600e\u4e48\u5b9a\u91cf\u7684\uff1f \u5408\u6210 DNA \/ RNA \u7684 OD \u503c\u5982\u4f55\u6d4b\u5b9a\u5e76\u8ba1\u7b97\uff1f \u5408\u6210\u7684 DNA \u5e72\u7c89\u5982\u4f55\u7a00\u91ca\u548c\u4fdd\u5b58\uff1f \u5408\u6210\u7684 RNA \u5e72\u7c89\u5982\u4f55\u7a00\u91ca\u548c\u4fdd\u5b58\uff1f \u5c0f\u4e8e 15 mer \u7684 Oligo DNA \/ RNA"},"footnotes":""},"class_list":["post-2555","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/en.agbio.com.cn\/en\/wp-json\/wp\/v2\/pages\/2555","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/en.agbio.com.cn\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/en.agbio.com.cn\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/en.agbio.com.cn\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/en.agbio.com.cn\/en\/wp-json\/wp\/v2\/comments?post=2555"}],"version-history":[{"count":0,"href":"https:\/\/en.agbio.com.cn\/en\/wp-json\/wp\/v2\/pages\/2555\/revisions"}],"wp:attachment":[{"href":"https:\/\/en.agbio.com.cn\/en\/wp-json\/wp\/v2\/media?parent=2555"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}