OK, Clon. The DNA Ligation Kit II is an accurate, fast and efficient targeted cloning kit. The kit allows for the rapid and efficient targeted and seamless cloning of one or more DNA fragments into any position of any vector in less than 10 minutes, without being constrained by the enzyme cleavage site. This product is available in OK, Clon. The DNA Ligation Kit (Code. AG11802 / AG11803) has been optimized for a wider range of applications, especially for the ligation of large vectors, large fragments and multiple fragments. OK, Clon. The exonuclease activity of the 3’-5’ end of the Enzyme cuts the nucleotide from the 3’ end of the linearized DNA strand to form a sticky end, thus the fragment can form a 15-20 bp complementary sequence with the end of the vector, and the fragment can be accurately and efficiently ligated to the vector by annealing. When designing the primer, only the sequence complementary to the 5’ end of the vector is added to the 5’ end of the primer, and the target fragment can be amplified by this primer, and it can be inserted into the vector without the need of restriction enzyme cutting or phosphorylation of the PCR fragment.
1. Fragments can be inserted into any position of the vector without being restricted by the enzyme cutting site;
2. Rapid reaction, 50 ℃ 10 min can complete the connection reaction;
3. Wider scope of application, applicable to all sizes of vector and fragment ligation; especially suitable for large vectors, large fragments and multi-fragment ligation;
4. The inserted fragments do not need to be digested and can be directly ligated after PCR amplification by purification.
| individual parts making up a compound | AG11807 | AG11808 |
| 2.5 X OK, Clon. Master Mix | 40 μl | 100 μl x 2 pc |
Storage temperature: -20℃ storage
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
