The DH5α receptor cells produced by our company are prepared by E.coli DH5α strain through a special process, which can be used for DNA chemical transformation; DH5α strain lacks non-specific nucleic acid endonuclease I activity, which can improve the yield and quality of plasmid DNA; DH5α strain belongs to recombinase-deficient strains, which can reduce the probability of recombination of the cloned DNA, and ensure the stability of the inserted DNA; when using pUC series plasmid vector for DNA transformation, the β-galactosidase activity (α-complementarity) can be utilized to identify recombinant strains by blue and white spotting screening. When using pUC series plasmid vectors for DNA transformation, β-galactosidase activity (α-complementarity) can be used to identify recombinant strains by blue and white spot screening. Since the strain has no lacIqIPTG is not required, and the transformation efficiency is > 1 × 10 using the pUC19 plasmid DNA assay.8 cfu/ug.
| individual parts making up a compound | norm |
| E. coli DH5α Competent cells* | 100 μl x 10 pc |
| SOC Medium | 1 ml × 10 pc |
| pUC19 plasmid (0.1 ng / μl) | 10 μl |
Suitable for plasmid transformation, gene cloning, blue and white spot screening and other uses.
Storage temperature: -80℃
Transportation temperature: dry ice transportation
