2X Multi PCR Premix (with dye)

Product Code: AG12309

¥900

Product Description

Multiplex DNA Polymerase is a 2-fold pre-mixed multiplex PCR product that can simultaneously detect multiple genes in a single PCR reaction system, and is suitable for multiplex PCR amplification of DNA fragments up to 2 kb. Multiplex DNA Polymerase lacks the 5’→3’ nucleic acid exonuclease activity of wild-type Taq, and is highly sensitive, efficient and specific. Multiplex DNA Polymerase lacks the 5'→3' exonuclease activity of wild-type Taq and is characterized by high sensitivity, high amplification efficiency and high specificity.
This product contains the necessary pigment reagent for electrophoresis, and the solution of the product is purple-red in color; when using this product, it is only necessary to add the template, primer and water to the solution to carry out the PCR reaction; after the PCR reaction, electrophoresis can be carried out directly, which is easy to operate, minimizing the human error, and the test results can be obtained in a relatively short period of time. In addition, monoclonal antibody that can inhibit the activity of DNA Polymerase at room temperature is added to this product, which can perform Hot Start PCR and effectively inhibit the formation of primer dimer and non-specific amplification.

Product Advantages

① This product is characterized by high sensitivity, high amplification efficiency and high specificity.
② Wide range of applications, capable of amplifying multiplexed PCRs with different template types, at least 20-fold DNA fragments can be amplified using Human gDNA as template, and at least 9-fold DNA fragments can be amplified using λDNA as template.
③ The product is a 2X premix, adding template, primer and water to carry out the PCR reaction, easy to operate, and can minimize human error.
This product contains the color reagent (purple) necessary for electrophoresis, and can be used for electrophoresis directly after the PCR reaction, so that test results can be obtained in a short time.

Product Composition

individual parts making up a compound	norm
2X Multiplex PCR Master Mix (dye plus)	625 μl x 2 pc

Preservation and transportation

Storage temperature: -20℃
Transportation temperature: dry ice or -20℃ ice bag transportation

Example 1

Using Human gDNA as template, 20-fold PCR amplification (70 bp ~ 697 bp) was performed with this kit, and up to 20 target fragments were successfully amplified.

Reaction Procedures:

The electrophoresis results are shown below:

Example 2

Using λDNA as a template, different template amounts (5 ng, 1 ng, 100 pg, 10 pg, 100 fg) were added, and the kit was used to amplify seven repetitions of DNA fragments (150 bp, 250 bp, 400 bp, 535 bp, 750 bp, 948 bp, and 1,500 bp) with a template amount as low as 100 fg, which resulted in the amplification of the target fragments.

Reaction Procedures: