2X <i>Accurate Taq</i> HS PCR 预混液（含 UNG）

2X Accurate Taq HS PCR Premix (with UNG)

Product Code: AG11210

- +

¥420

Product Description

The dUTP/UNG Anti-Contamination System is introduced in this product, which replaces dTTP with dUTP in the PCR reaction, and utilizes UNG enzyme to selectively hydrolyze dU-containing DNA strands without affecting dU-free DNA strands to remove dU-containing contaminating templates introduced in the preparation of the PCR reaction system, thus effectively preventing the generation of false-positive results of the PCR and enhancing the accuracy of the results. This can effectively prevent the generation of false-positive PCR results and improve the accuracy of the results.
This product also contains an additive that can inhibit the production of chemical substances at room temperature. Accurate Taq An active monoclonal antibody that enables Hot Start PCR and effectively inhibits primer dimer formation and non-specific amplification. The PCR product obtained with this product has an A base at the 3’ end and can be cloned directly into T vectors.
At the same time, this product is a 2X PCR reaction premix, only need to add primers, templates and water to the premix to carry out the PCR reaction, the operation is simple, can minimize human error, and can be obtained in a relatively short period of time.

Product Advantages

1. dUTP/UNG is a contamination prevention system that removes contamination of dU-containing PCR products to prevent false-positive PCR results and improve the accuracy of the results.
2、This product adopts the superior performance of the Accurate Taq HS and optimized reaction system can effectively inhibit non-specific amplification.
3、This product is a 2X premix, the reaction solution is very simple to prepare, only need to add primers, templates and water to carry out the PCR reaction.

Product Composition
individual parts making up a compound AG11210
2X Accurate Taq HS PCR Master Mix (UNG plus) 1 ml x 5 pcs
RNase free water 1 ml x 5 pc
Preservation and transportation
Storage temperature: -20℃
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
Example 1

Using 100 ng of HL60 gDNA as a template, the product was used to amplify a 500 bp fragment of DNA, and RNase free water was used instead of 2X. Accurate Taq HS PCR Master Mix (UNG plus) was used for PCR amplification as a residual template control group (first round of PCR amplification). In the second round of PCR amplification, 2 μl of the first round of PCR amplification product was used as the template for the second round of PCR amplification. The second round of PCR amplification was divided into three groups: Group 1 was untreated, and the product obtained from the first round of PCR amplification using the product was used as the template; Group 2 was inactivated by the reagent with UNG (95℃ for 2 min), and the product obtained from the first round of PCR amplification using the product was used as the template; Group 3 reagents were not treated, and the residual template product obtained in the first round of PCR amplification using RNase free water was used as the template; the amplification method was the same as that of the first round of PCR amplification, and the difference in the results confirmed the template elimination ability of this product.

The electrophoresis results are shown below:

Example 2

Using λDNA as a template, this product can amplify DNA fragments of about 10 kb.

The electrophoresis results are shown below:

Example 3

Using HL 60 gDNA as a template, the product amplifies a DNA fragment of approximately 3.4 kb, resulting in a bright and single target band.

The electrophoresis results are shown below:

Example 4

Using HL 60 gDNA as a template and different template amounts (200 ng, 100 ng, 10 ng, 1 ng), the product amplified approximately 1 Kb of DNA fragments, and the target bands could still be amplified at template amounts as low as 1 ng.

The electrophoresis results are shown below:

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