Calcium phosphate-mediated transfection is one of the most widely used methods to introduce exogenous DNA into cells, based on the principle that in phosphate-containing HEPES buffer, plasmid DNA and calcium ions form a DNA/calcium phosphate precipitate, which is adsorbed on the cell surface and taken up by the cell through cytotranspiration; the transfected DNA can be transiently expressed inside the cell, and also can be integrated into the genome of the target cell to produce stable clones. The transfected DNA can be transiently expressed in the cell or integrated into the genome of the target cell to produce a stable clone. This method is simple and suitable for plasmid DNA transfection in a variety of mammalian adherent cells.
①The pH value and salt ion concentration of this product are highly stable, which ensures a high degree of consistency in transfection efficiency;
②This product is not only suitable for transient transfection of cells, but also for stable transfection of cells;
③This product is suitable for most of the adherent cells.
| individual parts making up a compound | norm |
| Calcium Solution | 1.25 ml × 4 pcs |
| 2X Hepes Buffered Saline (HBS)* | 10 ml × 4 pcs |
| Sterile Water | 9 ml × 4 pcs |
* 2X Hepes Buffered Saline (HBS) should avoid repeated freezing and thawing. It is recommended that after the initial thawing, it be dispensed according to experimental requirements and stored at -20°C.
Transportation temperature: dry ice transportation or -20℃ ice bag transportation.
