This product uses environmentally friendly dewaxing method to remove paraffin wax, which is safe and non-toxic. Small RNA (including miRNA, siRNA, tsRNA and piRNA, etc.) can be extracted from 3~10 paraffin sections of 5~10 μm thickness, paraffin blocks of less than 30 mg and formalin-fixed tissue samples of less than 30 mg. An optimized lysis system was used to release RNA from the paraffin samples, overcoming the inhibitory effect caused by formalin cross-linking. By removing impurities such as protein, gDNA and large fragment RNA through subsequent purification, high purity Small RNA can be obtained. Small RNA extracted with this product is of high purity and stable quality, and can be used in downstream experiments such as RT-qPCR and NGS.
| individual parts making up a compound | norm |
| Proteinase K (20 mg/ml) | 1 ml |
| DNase I (RNase free) | 200 μl |
| 10X DNase I Buffer | 1 ml |
| Buffer DP | 30 ml |
| Buffer RLS-2 for small RNA | 13 ml |
| Buffer RBS-2 | 11 ml |
| Buffer RWA for small RNA*2 | 14 ml |
| Buffer RWB*2 | 27 ml |
| RNase Free Water*3 | 10 ml |
| FFPF Small RNA Mini Columns | 50 sets X 2 pcs |
| Collection tubes | 50 pcs |
| RNase Free Tubes*4 | 50 pcs |
*1: Proteinase K (20 mg / ml), DNase I (RNase free) and 10X DNase I Buffer are packaged in Package 2-1 and should be stored at -20℃; the rest of the components are packaged in Package 2-2 and should be stored at room temperature (15 ~ 30℃).
*2: Before using Buffer RWA for small RNA for the first time, please add 21 ml of anhydrous ethanol (the volume ratio of Buffer RWA for small RNA to anhydrous ethanol is 2:3), and before using Buffer RWB for the first time, please add 63 ml of anhydrous ethanol (the volume ratio of Buffer RWB to anhydrous ethanol is 3:7). After mixing well, mark the bottle and store it at room temperature.
*3: RNase Free Water is recommended to be stored at -20°C after opening.
*4: RNase Free Tubes are for elution of RNA only. Centrifuge tubes for pre-lysis should be provided.
Preservation Temperature:
Package 2-1 -20°C Storage
Package 2-2 Room Temperature (15-30°C) Storage
Transportation temperature:
Package 2-1 -20℃ Ice bag transportation or dry ice transportation
Package 2-2 Room Temperature Transportation
