Product Description

ApexDirect DNA Polymerase is a high performance DNA polymerase with excellent amplification properties and wide template applicability for routine PCR amplification. It is also resistant to inhibitors and is particularly suitable for direct PCR of blood, animal and plant tissues. It can be used to amplify templates with high GC content, high AT content and low starting volume.

Product Advantages

① ApexDirect DNA Polymerase is a high performance DNA polymerase with excellent amplification properties and broad template applicability.
② This product is very resistant to inhibitors and is particularly suitable for direct PCR reactions on blood, animal and plant tissues, and so on.
③ The optimized reaction system is well adapted to PCR amplification of complex templates, and it can effectively amplify templates with high GC content, high AT content and low starting amount.

Product Composition

individual parts making up a compound	norm
ApexDirect DNA Polymerase (1 U/μl)	100 μl
2X ApexDirect Buffer (Mg)²⁺ and dNTP plus)*	1.25 ml × 2 pcs

* ApexDirect Buffer (Mg)²⁺ and dNTP plus) in the Mg²⁺concentration of 4 mM and dNTP concentration of 800 μM each.

Preservation and transportation

Storage temperature: -20℃
Transportation temperature: dry ice transportation or -20℃ ice bag transportation

Example 1

This kit was used to amplify DNA fragments with different GC contents, and it amplified both high GC and high AT DNA fragments very well.

The electrophoresis results are shown below:

Example 2

Using Human gDNA as template and adding different template amounts (500 ng, 200 ng, 100 ng, 10 ng, 1 ng, 100 pg), a 2 kb DNA fragment can be amplified with 100 pg template amount using this kit.