ApexHF HS DNA Polymerase Premix - FCL (with dye)

Product Code: AG12212

norm

100 rxns / 50 μl

Clear

- +

¥850

Product Overview

ApexHF HS DNA Polymerase FCL Master Mix (dye plus) is an optimized 2-fold concentration of high-fidelity enzyme premix that combines amplification success and amplification speed. It is characterized by high amplification efficiency, high specificity, high fidelity, high annealing efficiency and fast amplification. The fastest amplification speed is up to 1 sec/kb for fragments up to 2 kb, and the longest amplification speed is up to ~40 kb, and it can effectively amplify templates with high GC, high AT content and low starting amount; the product contains color reagents necessary for electrophoresis. The product contains the necessary pigment reagents for electrophoresis, so when performing the PCR reaction, only the template and primers need to be added to the pre-mixed solution to carry out the amplification, and after the reaction is completed, it can be directly carried out on agarose gel electrophoresis, which can minimize the human error and obtain the test results in a shorter period of time.
In addition, a monoclonal antibody that inhibits the activity of DNA polymerase at room temperature has been added to this product, which enables Hot Start PCR and effectively inhibits the formation of primer dimers and non-specific amplification.

Product Advantages

1. Fast extension speed: 1 sec/kb for fragments up to 2 kb, 5 sec/kb for fragments from 2 to 6 kb, and 10 sec/kb for fragments from 6 to 10 kb.
2. Wide applicability of templates: suitable for all kinds of refined samples, compatible with coarse templates of animal and plant lysate, blood, bacterial fluids and so on.
3. Suitable for amplification of long fragments: 40 kb of DNA fragments using λDNA as a template; approximately 32 kb of DNA fragments using Human gDNA as a template.
4. Optimized reaction system with good adaptability to simple or complex templates, short fragments or long fragments PCR amplification. Efficient amplification of templates with high GC content, high AT content and low starting volume.
5. A monoclonal antibody capable of inhibiting DNA polymerase activity has been added, which binds to the enzyme to inhibit DNA polymerase activity prior to the PCR reaction, effectively inhibiting the formation of primer dimers and non-specific amplification.
6. This product is a 2X premix, which is easy to use for PCR amplification by adding only the template and primers.
7. This product contains an electrophoresis indicator, which can be used for electrophoresis directly after the PCR reaction is completed.

Product Composition

individual parts making up a compound	norm
ApexHF HS DNA Polymerase FCL Master Mix (dye plus)	500 μl X 5 pcs

Preservation and transportation

Storage temperature: -20℃ storage
Transportation temperature: dry ice transportation or -20℃ ice bag transportation

test case

1. Using human genomic DNA as a template, this product can be used to amplify DNA fragments of different lengths at different extension speeds.

Reaction Procedure.

The electrophoresis results are shown below:

2. Using Human gDNA and λDNA as templates, the product can amplify DNA fragments of different lengths, and can amplify DNA fragments of 32 kb and 40 kb respectively.

Reaction Procedure.

The electrophoresis results are shown below:

3. Using Human, Mouse and Chicken gDNA as templates, this product can amplify DNA fragments with different GC contents at a speed of 5 sec/kb, and can amplify DNA fragments with high GC and high AT very well.

Reaction Procedure.

The electrophoresis results are shown below:

4. Using Human gDNA as template and adding different template amounts (500 ng, 200 ng, 100 ng, 10 ng, 1 ng, 100 pg), the product can amplify 4 kb DNA fragments, and can amplify DNA fragments with 100 pg template amount.

Reaction Procedure.

The electrophoresis results are shown below:

5. The samples such as rat tail and rice leaf were simply lysed by proteinase K method [using our product Lysis Buffer for PCR (Code No. AG12306)], and 2 μl of the simple lysate was taken and amplified with the product, and both of them got good amplification results; meanwhile, 2 μl of the human blood sample and the sample of E. coli bacterial fluid were amplified directly with the product, and the amplification effect could be obtained very well. At the same time, the direct amplification of 2 μl of human blood and E. coli bacterial samples using this product also achieved good amplification results.

Reaction Procedure.