2X L-Exp Taq Premix (with dyes)

Product Code: AG11418

- +

¥650

Product Description

This product is ready to use L-Exp Taq Enzyme PCR reaction 2-fold pre-mix containing L-Exp Taq DNA Polymerase, dNTPs and optimized Buffer system, PCR reaction, just add template, primer and water to the premixed solution for amplification. At the same time, the coloring reagent required for electrophoresis is added to the product, and the reaction solution appears purple-red, which can be used for agarose gel electrophoresis directly after PCR. This premix solution is easy to operate, can minimize human error, reduce the possible contamination caused by multi-step operation, and the test results can be obtained in a short time.
This product is made of our superior performance Accurate Taq The DNA PCR reaction system is optimized for PCR amplification of long and complex DNA sequences with high fidelity and the 3’ end of the PCR product has an A base for direct cloning into T-vectors. The PCR product has an A base at the 3' end and can be cloned directly into T vectors.

Product Advantages
① The product is a 2X premix, only need to add template, primer and water to the premix for amplification, easy to operate, can minimize human error, reduce the possible contamination caused by multi-step operation, and the results can be obtained in a shorter period of time.
② 2X L-Exp Taq The Master Mix (dye plus) amplifies ~40 kb of DNA fragments using λDNA as a template and ~24 kb of DNA fragments using Human genomic DNA as a template.
③ Suitable for amplification of complex templates such as DNA fragments with high GC content.
④ This product contains violet dye, after PCR can be directly carried out agarose gel electrophoresis, without adding electrophoresis sample buffer, electrophoresis has a light violet-red indicator band.
Product Composition
individual parts making up a compound norm
2X L-Exp Taq Master Mix (dye plus) 500 μl / 6 pc
RNase free water 1 ml x 3 pc
Preservation and transportation
Storage temperature: -20℃
Transportation temperature: dry ice or -20℃ ice bag transportation
Example 1

Using λDNA as a template, the kit amplifies DNA fragments of different lengths, and is able to amplify 40 kb of DNA fragments.

The electrophoresis results are as follows:

Example 2

Using human gDNA as a template, this kit amplifies DNA fragments of different lengths, and can well amplify 24 kb DNA fragments.

The electrophoresis results are as follows:

Example 3

pick E. coli The single colony was added to 20 μl of RNase free water, mixed well, and then 1 μl of the bacterial solution was used as a template to amplify DNA fragments of different lengths using this kit, and 11 kb of DNA fragments were well amplified.

The electrophoresis results are as follows: