Product Description
This product is ready to use L-Exp Taq Enzyme PCR reaction 2-fold pre-mix containing L-Exp Taq DNA Polymerase, dNTPs, and optimized Buffer systems are used to amplify the PCR reaction by simply adding template, primers, and water to the premix. This premix solution is easy to use, minimizes human error, reduces contamination caused by multiple steps, and results can be obtained in a short period of time.
The PCR reaction system has been carefully optimized to provide excellent amplification of long fragments and complex templates, with amplification of ~24 kb of DNA fragments using Human genomic DNA as a template, and high success rates with difficult-to-amplify templates with high GC content (~75%). The PCR products obtained with this product have an A base at the 3’ end and can be cloned directly into T vectors.
The PCR reaction system has been carefully optimized to provide excellent amplification of long fragments and complex templates, with amplification of ~24 kb of DNA fragments using Human genomic DNA as a template, and high success rates with difficult-to-amplify templates with high GC content (~75%). The PCR products obtained with this product have an A base at the 3’ end and can be cloned directly into T vectors.
Product Advantages
① The product is a 2X premix, only need to add template, primer and water to the premix for amplification, easy to operate, can minimize human error, reduce the possible contamination caused by multi-step operation, and the results can be obtained in a relatively short period of time.
② 2X L-Exp Taq Master Mix has good amplification performance. It can amplify ~40 kb of DNA fragments using λDNA as template and ~24 kb of DNA fragments using Human genomic DNA as template.
② 2X L-Exp Taq Master Mix has good amplification performance. It can amplify ~40 kb of DNA fragments using λDNA as template and ~24 kb of DNA fragments using Human genomic DNA as template.
③High-fidelity enzyme is added to this product, relative to the Accurate Taq Has higher fidelity and stronger amplification ability. High amplification efficiency and low mismatch rate under normal PCR amplification conditions.
Product Composition
| individual parts making up a compound | norm |
| 2X L-Exp Taq Master Mix | 500 μl x 6 pc |
| RNase Free Water | 1 ml x 3 pc |
Preservation and transportation
Storage temperature: -20℃
Transportation temperature: dry ice or -20℃ ice bag transportation
Transportation temperature: dry ice or -20℃ ice bag transportation
