AccuNext Single Cell Whole Genome Amplification Kit

This product is a Whole Genome Amplification (WGA) kit specially developed for micro samples such as 1~10 Cells or 15 pg~100 pg genomic DNA. The optimized enzyme and buffer system can complete the steps of cell lysis, pre-amplification and amplification within 3 hours, and obtain microgram amplification products. The product is easy to operate, and the amplified products have good homogeneity and high reproducibility, which can be widely used in whole genome sequencing, exon sequencing, copy number variation (CNV) analysis, single nucleotide variation (SNV) analysis, qPCR analysis, gene microarray analysis, and pre-implantation genetic screening (PGS) applications.

1. Ultra-high sensitivity: Uniform whole-genome amplification using single cells or trace amounts of DNA as templates.
2. The optimized reaction system is simple to operate in a single tube, and can obtain uniform and highly reproducible microgram amplification products within 3 hours.
3. Single-tube operation, the whole process does not require purification and tube transfer, reducing the sample loss and contamination caused by excessive operation, ensuring the accuracy of experimental data.
4. With the characteristics of good amplification uniformity and high sensitivity, it can be widely used in whole genome and exon sequencing, copy number variation (CNV) analysis, single nucleotide variation (SNV) analysis, qPCR analysis, gene chip analysis, and preimplantation genetic screening (PGS).

individual parts making up a compound	AG12553	AG12554
Cell Lysis Buffer	60 μl	240 μl
Cell Lysis Enzyme	5 μl	15 μl
AccuNext  Pre-Amp Buffer	96 μl	384 μl
AccuNext  Pre-Amp Enzyme	24 μl	96 μl
AccuNext  Amplification Buffer	300 μl	1.2 ml
AccuNext  Amplification Enzyme	36 μl	144 μl
Nuclease free water	1 ml	1 ml

 

Storage temperature: -20℃ storage
Transportation temperature: -20℃ ice bag transportation