Product Description
This product is a transposase DNA library kit developed for illumina's high-throughput sequencing platform, which can prepare a sequencing library from a purified 1 ng DNA sample. Using a novel transposase and optimized buffer system, the product can complete DNA fragmentation and junction ligation in only 10 min, which is much simpler and more convenient than the traditional library preparation method, eliminating the need for cumbersome DNA fragmentation, end repair and junction ligation reactions, and significantly shortening the time for library construction. Since Transposome Mix V1 is very sensitive to the amount of DNA added, the exact amount of DNA added is critical to the success of the experiment, so it is recommended to add 1 ng of DNA template without adjustment. For other template incorporation amounts, 2 other kits with different template starting amounts are available, which are suitable for DNA template incorporation of 50~100 ng (CodeNo. AG12518/AG12519) and 5 ng (CodeNo. AG12522/AG12523), respectively.
The reaction system of this product has been carefully optimized, all reagents in the experiment should be used as provided in this product, it is not recommended to change the dosage and concentration of any reaction components or to replace the components in this product with other equivalent products in order to avoid obtaining bad results. If replacement is needed, please verify it first.
The reaction system of this product has been carefully optimized, all reagents in the experiment should be used as provided in this product, it is not recommended to change the dosage and concentration of any reaction components or to replace the components in this product with other equivalent products in order to avoid obtaining bad results. If replacement is needed, please verify it first.
Product Advantages
1. Using a new transposase and optimized reaction system, DNA fragmentation and ligation steps can be completed in only 10 min, which significantly shortens the library construction time, and library construction can be completed in less than 1.5 hours, and the library quality is excellent.
2. Simple operation, single-tube enzymatic reaction for fragmentation and splicing without mechanical interruption.
3. Low DNA template incorporation, down to 1 ng of DNA template.
4. Wide applicability of templates, 4 different template quantities (50~100 ng / 5 ng / 1 ng) have been designed and can be selected according to the needs.
2. Simple operation, single-tube enzymatic reaction for fragmentation and splicing without mechanical interruption.
3. Low DNA template incorporation, down to 1 ng of DNA template.
4. Wide applicability of templates, 4 different template quantities (50~100 ng / 5 ng / 1 ng) have been designed and can be selected according to the needs.
Product Composition
| individual parts making up a compound | AG12524 | AG12525 |
| AccuNext Transposome Mix V1*a | 24 μl | 96 μl |
| 5X Tn5 Reaction Buffer*b | 48 μl | 192 μl |
| 5X Stop Buffer*b | 60 μl | 240 μl |
| AccuNext PCR Mix II*b | 240 μl | 960 μl |
| Nuclease free water | 1 ml | 1 ml X 2 pcs |
*a:AccuNext Transposome Mix V1 should be stored at -80°C; for 1 ng of DNA template, do not adjust the amount of template added.
*b: 5X Tn5 Reaction Buffer, 5X Stop Buffer and AccuNext PCR Mix can be stored at -80°C or -20°C.
*c: illumina sequencing junction primers for PCR amplification are required for the experiment but are not configured in this product and need to be purchased separately. [For example, the AccuNext Transposome building library adaptor primers (Illumina) (Code No. AG12532, AG12533, AG12534)].
Preservation and transportation
Storage temperature: -80℃ storage
Transportation temperature: dry ice transportation
Transportation temperature: dry ice transportation
