ApexHF HS DNA Polymerase Premix - FS (with dye)

Name: ApexHF HS DNA 聚合酶预混液 - FS（含染料）
SKU: 866
Availability: InStock

Product Code: AG12206

norm

100 rxns / 50 μl

Clear

- +

¥400

Product Description

This product is ApexHF HS DNA Polymerase FS is a ready-to-use 2-fold PCR reaction premix. For PCR reaction, just add template, primer and water to the premix for amplification; meanwhile, the coloring reagent (light purple-red) for electrophoresis is also added to this product, so that gel electrophoresis can be carried out directly after the PCR reaction, and the reaction solution will appear purple-red. This premix solution is easy to operate, can minimize human error, and the results can be obtained in a short period of time. The product is characterized by high amplification efficiency, high sensitivity, high specificity, high annealing efficiency and fast extension speed, which can be used for rapid PCR reaction. In addition, monoclonal antibody that can inhibit the activity of DNA polymerase at room temperature has been added to the product, which can be used for Hot Start PCR and effectively inhibit the formation of primer dimer and non-specific amplification.

Product Advantages

① ApexHF HS DNA Polymerase FS is a high-fidelity DNA polymerase with high amplification efficiency, high sensitivity, high specificity, high annealing efficiency and fast extension speed to improve PCR reaction efficiency.
② The extension speed of this product is fast, using Human gDNA as template, 10 sec extension time, can amplify DNA fragments up to 4 kb.
③ A monoclonal antibody capable of inhibiting DNA polymerase activity has been added to this product, which can be used for hot start reaction. Before the PCR reaction, the antibody binds to the enzyme and inhibits the activity of DNA polymerase, effectively inhibiting the formation of primer dimers and non-specific amplification.

This product is a 2X premixed solution, which can be used for PCR amplification by adding template, primer and water, which is easy to operate and reduces human error.

⑤ The system contains an electrophoretic indicator, which can be used for electrophoresis directly after the PCR reaction is completed, and there is only one indicator band, which is light purple-red in color during electrophoresis.

Product Composition

individual parts making up a compound	norm
2X ApexHF FS PCR Master Mix (dye plus)*	500 μl x 5 pcs

* Mg in solution²⁺concentration was 2 mM and the concentration of dNTPs was 400 μM .

Preservation and transportation

Storage temperature: -20℃
Transportation temperature: dry ice transportation or -20℃ ice bag transportation

Example 1

Human gDNA was used as the template to amplify DNA fragments of different lengths at different extension speeds. 4 kb of DNA fragments were well amplified with an extension time of 10 sec.

The electrophoresis results are shown below:

Example 2

Using cDNA as a template and adding different template amounts (equivalent to the amount of Total RNA), this kit is used to amplify 4 kb DNA fragments. When rapid amplification is performed (extension time of 20 sec), with a template amount of 5 ng (equivalent to the amount of Total RNA), the target fragment can be amplified; when the extension time is prolonged, the amount of DNA amplified will increase, and at the same time, a high template amount will also be able to The amount of DNA amplified will increase when the extension time is extended, and at the same time, a high template amount can be well amplified.

The electrophoresis results are shown below:

Example 3

Using Human gDNA as template, different template amounts (500 ng, 300 ng, 100 ng, 10 ng, 1 ng, 100 pg) were added to amplify 4 kb DNA fragments, and with template amounts as low as 1 ng, the target fragments could be amplified at a rate of 5 sec/kb.

The electrophoresis results are shown below:

Example 4

Using λDNA as template, different template amounts (5 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg) were added to amplify 4 kb DNA fragments, and the target fragments were amplified at 5 sec/kb at template amounts as low as 100 fg.