Exp Taq DNA Polymerase Ver.2 (Mg)2++, dNTPs+)

Item No.: AG11403 / AG11411

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Price range: ¥210 through ¥760

Product Description
This product is a PCR reaction system carefully optimized by applying the principle of LA PCR, and is manufactured with our superior performance PCR system. Accurate Taq The addition of high-fidelity enzymes to the enzyme results in partial 3′-5′ Exonuclease activity (Proof reading activity), which is ideal for PCR amplification of long DNA fragments with good fidelity. Most of the PCR products obtained with this product have an A base at the 3’ end and can be cloned directly into T vectors.
Product Advantages
Exp Taq DNA Polymerase with the addition of high-fidelity enzymes relative to the Accurate Taq Has higher fidelity and stronger amplification ability. High amplification efficiency and low mismatch rate under normal PCR amplification conditions.
② This product can amplify ~40 kb of DNA fragments using λDNA as a template and ~20 kb of DNA fragments using Human genomic DNA as a template.
Product Composition
individual parts making up a compound AG11403 AG11411
Exp Taq DNA Polymerase (5 U/μl) 100 μl 25 μl
5X Exp Taq PCR Buffer Ver.2 (Mg)2+ plus) 1 ml x 2 pc 500 μl
dNTP Mix (10 mM each) 400 μl 100 μl
Preservation and transportation
Storage temperature: -20℃
Transportation temperature: dry ice or -20℃ ice bag
Example 1

Using Human gDNA as template, this kit amplifies DNA fragments of different lengths, and can well amplify 20 kb DNA fragments.

The electrophoresis results are shown below:

Example 2

Using λDNA as a template, the kit amplifies DNA fragments of different lengths, and is able to amplify 40 kb of DNA fragments.

The electrophoresis results are shown below:

Example 3

Using different template amounts (5 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg) of λDNA as template, the kit was used to amplify DNA fragments up to ~15 kb with a sensitivity of up to 100 fg.

The electrophoresis results are as follows: