多重 PCR 试剂盒

Multiplex PCR kits

Product Code: AG12308

- +

¥800

Product Description

Multiplex DNA Polymerase is a multiplex PCR kit for simultaneous detection of multiple genes in a single PCR reaction system and is suitable for multiplex PCR amplification of DNA fragments up to 2 kb.Multiplex DNA Polymerase lacks the 5’→3’ exonuclease activity of wild-type Taq. Multiplex DNA Polymerase lacks the 5'→3' nucleic acid exonuclease activity of wild-type Taq and is characterized by high sensitivity, high amplification efficiency and high specificity. In addition, a monoclonal antibody that inhibits the activity of DNA Polymerase at room temperature has been added to this product, which can be used for Hot Start PCR to effectively inhibit the formation of primer dimers and non-specific amplification.

Product Advantages

① This product is characterized by high sensitivity, high amplification efficiency and high specificity.
② Wide range of applications, capable of amplifying multiplexed PCRs with different template types, at least 20-fold DNA fragments can be amplified using Human gDNA as template, and at least 9-fold DNA fragments can be amplified using λDNA as template.
③ Good specificity, monoclonal antibody that can inhibit the activity of Multiplex DNA polymerase is added to this product, so it can carry out hot start reaction. Before PCR reaction, the antibody binds to the enzyme to inhibit the activity of Taq polymerase, effectively inhibiting the formation of primer dimer and non-specific amplification.

Product Composition
individual parts making up a compound norm
Multiplex DNA Polymerase (1 U/μl) 50 μl
2X Multiplex PCR Buffer (Mg)2+ and dNTP plus) 1.25 ml
Preservation and transportation
Storage temperature: -20℃
Transportation temperature: dry ice or -20℃ ice bag transportation
Example 1

Using Human gDNA as template, 20-fold PCR amplification (70 bp ~ 697 bp) was performed with this kit, and up to 20 target fragments were successfully amplified.

Reaction Procedures:

The electrophoresis results are shown below:

Example 2

Using λDNA as a template, different template amounts (5 ng, 1 ng, 100 pg, 10 pg, 100 fg) were added, and the kit was used to amplify seven repetitions of DNA fragments (150 bp, 250 bp, 400 bp, 535 bp, 750 bp, 948 bp, and 1500 bp) with the template amount as low as 100 fg.

Reaction Procedures:

The electrophoresis results are shown below:

Example 3

Human gDNA was used as the template, and different template amounts (500 ng, 100 ng, 10 ng, 1 ng) were added to amplify 6-fold (100 bp, 207 bp, 300 bp, 500 bp, 1005 bp, 1535 bp) DNA fragments using the kit, and the target DNA fragments were amplified at template amounts as low as 10 ng.

Reaction Procedures:

The electrophoresis results are shown below:

Example 4

Mouse gDNA was used as the template to amplify DNA fragments with different GC contents, and the target DNA fragments could be amplified. The procedure and template amount are the same for both single and multiplex.

Reaction Procedures:

The electrophoresis results are shown below:

Example 5

The rice gDNA was used as a template for 6-fold (94 bp-712 bp) amplification using different cycles of the kit, and the target DNA fragments were amplified in 35 cycles.

Reaction Procedures:

The electrophoresis results are shown below:

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