SteadyPure The Plant Genomic DNA Extraction Kit (CTAB Method) is designed to provide customers with a fast, simple, cost-effective, high-yield, high-quality method for the preparation of genomic DNA from plant samples, which is particularly suitable for the extraction of genomic DNA from complex plant genomes. It combines the classical CTAB method with silica gel column purification technology to extract high quality genomic DNA from less than 100 mg of fresh or frozen plant samples, as well as less than 30 mg of dried plant samples, which can be dissolved in water or Tris buffer and used directly for PCR amplification and DNA sequence analysis.
| individual parts making up a compound | norm |
| RNase A (10 mg/ml)*1 | 500 μl |
| Buffer LS-5 | 40 ml |
| Buffer WA | 25 ml |
| Buffer WB*2 | 27 ml |
| Elution Buffer | 10 ml |
| Plant DNA Mini Columns | 50 sets |
| Collection tubes | 50 pcs |
*1 RNase A (10 mg/ml) is packaged in Package 2-1 and should be stored at -20°C. The rest of the components are packaged in Package 2-2 and should be stored at room temperature (15 ~ 30°C).
*2 Before using Buffer WB for the first time, please add 63 ml of 100% ethanol (volume ratio of Buffer WB to 100% ethanol: 3:7), mix well, mark the bottle and store at room temperature.
Preservation Temperature:
Package 2-1 is stored at -20°C;
Package 2-2 Store at room temperature (15 °C ~ 30 °C).
Transportation temperature:
Package 2-1 Dry Ice Transportation or -20°C Ice Pack Transportation;
Package 2-2 Room Temperature (15 °C ~ 30 °C) Transportation.
