The kit can be used to rapidly extract RNA from a wide range of plant tissues (including roots, stems, leaves, flowers, fruits, seeds, etc. of simple or complex plants) from less than 100 mg. The kit uses a unique lysis system that eliminates the need for toxic phenol-chloroform extraction, and rapidly lyses tissues while inhibiting nuclease release from the cells to maintain RNA integrity, and is equipped with gDNA The Eraser Mini Columns effectively remove impurities and genomic DNA.Quick Plant RNA Mini Columns bind RNA efficiently, and the extraction process takes only 10 minutes after sample homogenization. The RNA obtained with this product has high purity and low contamination of proteins, genomic DNA and other impurities, and can be used directly for RT-PCR, Real Time RT-PCR, and RNA library construction,
Northern hybridization, in vitro translation, molecular cloning, microarray analysis and other molecular biology experiments.
| individual parts making up a compound | norm |
| Buffer QPLS | 30 ml |
| Buffer QWA | 30 ml |
| Buffer QWB*1 | 30 ml |
| RNase Free Water*2 | 10 ml |
| gDNA Eraser Mini Columns | 50 sets |
| Quick Plant RNA Mini Columns | 50 sets |
| Collection tubes | 50 pcs |
| RNase Free Tubes*3 | 50 pcs |
*1: Before using Buffer QWB for the first time, please add 70 ml of anhydrous ethanol (the volume ratio of Buffer QWB to anhydrous ethanol is 3:7), mix it well, mark the bottle and store it at room temperature.
*2: RNase Free Water is recommended to be stored at -20°C after opening.
*3: This component is only used to elute RNA, the centrifuge tube used for pre-lysis should be provided.
Storage temperature: room temperature (15~30℃) storage
Transportation temperature: room temperature
