SteadyPure The Plasmid DNA Extraction Kit is designed to provide customers with a fast, simple and cost-effective method of preparing small amounts of plasmid. The kit uses an optimized SDS-alkaline lysis method to lyse cells and a highly efficient centrifugal adsorption column to obtain high-quality, high-purity plasmid DNA, which can be directly used for subsequent transformation, DNA sequence analysis, in vitro transcription, restriction enzyme digestion, and a variety of other enzymatic reactions.
| individual parts making up a compound | AG21001 | AG21002 |
| RNase A (10 mg/ml) | 140 μl | 700 μl |
| Buffer RS*2 | 14 ml | 70 ml |
| Buffer LS | 14 ml | 70 ml |
| Buffer BS | 18 ml | 90 ml |
| Buffer WA | 25 ml | 125 ml |
| Buffer WB *3 | 27 ml | 135 ml |
| Elution Buffer | 10 ml | 20 ml |
| Plasmid DNA Mini Columns | 50 sets | 125 sets x 2 |
| Collection tubes | 50 pcs | 125 pcs x 2 |
*1 component RNase A is packaged in Package 2-1 and should be stored at -20℃; the rest of the components are packaged in Package 2-2 and stored at room temperature (15 - 25℃).
*2 Before the first use of Buffer RS, mix RNase A into Buffer RS (RNase A to Buffer RS volume ratio 1:100), mix evenly and label the bottle. Buffer RS with RNase A should be stored at 2-8°C for 6 months.
*3 Before using Buffer WB for the first time, please add 63 ml of anhydrous ethanol (Buffer WB to anhydrous ethanol volume ratio of 3:7), mix well, mark the bottle and store at room temperature.
Preservation Temperature:
Package 2-1 -20°C Storage
Package 2-2 Storage at room temperature (15-25°C)
Transportation temperature:
Package 2-1 Dry Ice Transportation or -20°C Ice Pack Transportation
Package 2-2 Room Temperature Transportation
