This product can extract up to 70 μg of plasmid DNA from 5 ml - 15 ml (<40 OD) of culture broth overnight.This product adopts the optimized SDS - alkaline lysis method to lysed cells, which can effectively remove impurities such as genomic DNA, RNA, and proteins, etc., and meanwhile, it can obtain high yield of plasmid DNA by using the highly efficient centrifugal adsorption column. The purified plasmid DNA can be directly used in subsequent molecular biology experiments such as transformation, DNA sequence analysis, in vitro transcription, restriction enzyme digestion, etc. The plasmid DNA can be directly used in subsequent molecular biology experiments such as transformation, DNA sequence analysis, in vitro transcription, restriction enzyme digestion, etc.
| individual parts making up a compound | norm |
| RNase A (10 mg/ml) | 280 μl |
| Buffer RS*2 | 28 ml |
| Buffer LS | 25 ml |
| Buffer BS | 35 ml |
| Buffer WA | 25 ml |
| Buffer WB*3 | 27 ml |
| Elution Buffer | 10 ml |
| Plasmid DNA Midi Columns | 50 sets |
| Collection tubes | 50 pcs |
*1 RNase A is packaged in Package 2-1 and should be stored at -20℃; the rest of the components are packaged in Package 2-2 and stored at room temperature (15 ~ 30℃).
*2 Before the first use of Buffer RS, RNase A should be mixed into Buffer RS (1:100 volume ratio of RNase A to Buffer RS), and the bottle should be labeled after mixing evenly. Buffer RS with RNase A should be stored at 2~8℃ for 6 months.
*3 Before using Buffer WB for the first time, please add 63 ml of anhydrous ethanol (the volume ratio of Buffer WB to anhydrous ethanol is 3:7), mix it well, mark the bottle and store it at room temperature.
Preservation Temperature:
Package 2-1 -20°C Storage
Package 2-2 Storage at room temperature (15 ~ 30°C)
Transportation temperature:
Package 2-1 Dry Ice or -20°C Ice Pack Transportation
Package 2-2 Room Temperature Transportation
